Monitoring of blood oxygenation in brain by resonance Raman spectroscopy

TY - JOUR

T1 - Monitoring of blood oxygenation in brain by resonance Raman spectroscopy

AU - Brazhe, Nadezda A.

AU - Thomsen, Kirsten

AU - Lønstrup, Micael

AU - Brazhe, Alexey R.

AU - Nikelshparg, Evelina I.

AU - Maksimov, Georgy V

AU - Lauritzen, Martin

AU - Sosnovtseva, Olga

N1 - This article is protected by copyright. All rights reserved.

PY - 2018/6

Y1 - 2018/6

N2 - Blood oxygenation in cerebral vessels is an essential parameter to evaluate brain function and to investigate the coupling between local blood flow and neuronal activity. We apply resonance Raman spectroscopy in vivo to study hemoglobin oxygenation in cortex vessels of anesthetized ventilated mice. We demonstrate that the pairs of Raman peaks at 1355 and1375 cm-1(symmetric vibrations of pyrrol half-rings in the heme molecule), 1552 and 1585 cm-1and 1602 and 1638 cm-1(vibrations of methine bridges in heme molecule) are reliable markers for quantitative estimation of the relative amount of oxyhemoglobin in venules, arterioles, and capillaries. In vivo measurements of blood oxygenation in the cortex of mice ventilated with inspiratory gas mixtures containing different amounts of oxygen - normoxia, hyperoxia and hypoxia - validate the proposed approach. Our method allows to visualize blood saturation with O2in different microvascular networks.

AB - Blood oxygenation in cerebral vessels is an essential parameter to evaluate brain function and to investigate the coupling between local blood flow and neuronal activity. We apply resonance Raman spectroscopy in vivo to study hemoglobin oxygenation in cortex vessels of anesthetized ventilated mice. We demonstrate that the pairs of Raman peaks at 1355 and1375 cm-1(symmetric vibrations of pyrrol half-rings in the heme molecule), 1552 and 1585 cm-1and 1602 and 1638 cm-1(vibrations of methine bridges in heme molecule) are reliable markers for quantitative estimation of the relative amount of oxyhemoglobin in venules, arterioles, and capillaries. In vivo measurements of blood oxygenation in the cortex of mice ventilated with inspiratory gas mixtures containing different amounts of oxygen - normoxia, hyperoxia and hypoxia - validate the proposed approach. Our method allows to visualize blood saturation with O2in different microvascular networks.

U2 - 10.1002/jbio.201700311

DO - 10.1002/jbio.201700311

M3 - Journal article

C2 - 29603883

SN - 1864-063X

VL - 11

JO - Journal of Biophotonics

JF - Journal of Biophotonics

IS - 6

M1 - e201700311

ER -