Molecular motor transport through hollow nanowires

Mercy Lard; Lasse Ten Siethoff; Johanna Generosi; Alf Månsson; Heiner Linke

doi:10.1021/nl404714b

Molecular motor transport through hollow nanowires

Mercy Lard^*, Lasse Ten Siethoff, Johanna Generosi, Alf Månsson, Heiner Linke

^*Corresponding author af dette arbejde

Kemisk Institut

25 Citationer (Scopus)

Abstract

Biomolecular motors offer self-propelled, directed transport in designed microscale networks and can potentially replace pump-driven nanofluidics. However, in existing systems, transportation is limited to the two-dimensional plane. Here we demonstrate fully one-dimensional (1D) myosin-driven motion of fluorescent probes (actin filaments) through 80 nm wide, Al₂O ₃ hollow nanowires of micrometer length. The motor-driven transport is orders of magnitude faster than would be possible by passive diffusion. The system represents a necessary element for advanced devices based on gliding assays, for example, in lab-on-a-chip systems with channel crossings and in pumpless nanosyringes. It may also serve as a scaffold for bottom-up assembly of muscle proteins into ordered contractile units, mimicking the muscle sarcomere.

Originalsprog	Engelsk
Tidsskrift	Nano Letters
Vol/bind	14
Udgave nummer	6
Sider (fra-til)	3041-3046
Antal sider	6
ISSN	1530-6984
DOI	https://doi.org/10.1021/nl404714b
Status	Udgivet - 2014

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10.1021/nl404714b

Citationsformater

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title = "Molecular motor transport through hollow nanowires",

abstract = "Biomolecular motors offer self-propelled, directed transport in designed microscale networks and can potentially replace pump-driven nanofluidics. However, in existing systems, transportation is limited to the two-dimensional plane. Here we demonstrate fully one-dimensional (1D) myosin-driven motion of fluorescent probes (actin filaments) through 80 nm wide, Al2O 3 hollow nanowires of micrometer length. The motor-driven transport is orders of magnitude faster than would be possible by passive diffusion. The system represents a necessary element for advanced devices based on gliding assays, for example, in lab-on-a-chip systems with channel crossings and in pumpless nanosyringes. It may also serve as a scaffold for bottom-up assembly of muscle proteins into ordered contractile units, mimicking the muscle sarcomere.",

keywords = "1D gliding assay, actin, Hollow nanowires, molecular motors, motor proteins, myosin",

author = "Mercy Lard and {Ten Siethoff}, Lasse and Johanna Generosi and Alf M{\aa}nsson and Heiner Linke",

year = "2014",

doi = "10.1021/nl404714b",

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journal = "Nano Letters",

issn = "1530-6984",

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TY - JOUR

T1 - Molecular motor transport through hollow nanowires

AU - Lard, Mercy

AU - Ten Siethoff, Lasse

AU - Generosi, Johanna

AU - Månsson, Alf

AU - Linke, Heiner

PY - 2014

Y1 - 2014

N2 - Biomolecular motors offer self-propelled, directed transport in designed microscale networks and can potentially replace pump-driven nanofluidics. However, in existing systems, transportation is limited to the two-dimensional plane. Here we demonstrate fully one-dimensional (1D) myosin-driven motion of fluorescent probes (actin filaments) through 80 nm wide, Al2O 3 hollow nanowires of micrometer length. The motor-driven transport is orders of magnitude faster than would be possible by passive diffusion. The system represents a necessary element for advanced devices based on gliding assays, for example, in lab-on-a-chip systems with channel crossings and in pumpless nanosyringes. It may also serve as a scaffold for bottom-up assembly of muscle proteins into ordered contractile units, mimicking the muscle sarcomere.

AB - Biomolecular motors offer self-propelled, directed transport in designed microscale networks and can potentially replace pump-driven nanofluidics. However, in existing systems, transportation is limited to the two-dimensional plane. Here we demonstrate fully one-dimensional (1D) myosin-driven motion of fluorescent probes (actin filaments) through 80 nm wide, Al2O 3 hollow nanowires of micrometer length. The motor-driven transport is orders of magnitude faster than would be possible by passive diffusion. The system represents a necessary element for advanced devices based on gliding assays, for example, in lab-on-a-chip systems with channel crossings and in pumpless nanosyringes. It may also serve as a scaffold for bottom-up assembly of muscle proteins into ordered contractile units, mimicking the muscle sarcomere.

KW - 1D gliding assay

KW - actin

KW - Hollow nanowires

KW - molecular motors

KW - motor proteins

KW - myosin

U2 - 10.1021/nl404714b

DO - 10.1021/nl404714b

M3 - Journal article

C2 - 24874101

AN - SCOPUS:84902250949

SN - 1530-6984

VL - 14

SP - 3041

EP - 3046

JO - Nano Letters

JF - Nano Letters

IS - 6

ER -

Molecular motor transport through hollow nanowires

Abstract

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