Mitochondrial oxidative phosphorylation capacity of cryopreserved cells

TY - JOUR

T1 - Mitochondrial oxidative phosphorylation capacity of cryopreserved cells

AU - Lauridsen, Poul Ejner

AU - Rasmussen, Lene Juel

AU - Desler, Claus

PY - 2019

Y1 - 2019

N2 - Defects in mitochondrial oxidative phosphorylation are a feature of many human diseases. To date, determination of oxidative phosphorylation has required fresh and live sample material and therefore also access to specialized equipment and trained personnel. Cryopreservation of samples is an attractive alternative, where samples can be collected and stored in an economic and practical fashion for later bulk assays. Here, we present an accurate, reliable method for estimating mitochondrial oxidative phosphorylation capacity of cryopreserved human cells. Broad adoption of this method will allow uncomplicated collection of samples and measurements of oxidative phosphorylation.

AB - Defects in mitochondrial oxidative phosphorylation are a feature of many human diseases. To date, determination of oxidative phosphorylation has required fresh and live sample material and therefore also access to specialized equipment and trained personnel. Cryopreservation of samples is an attractive alternative, where samples can be collected and stored in an economic and practical fashion for later bulk assays. Here, we present an accurate, reliable method for estimating mitochondrial oxidative phosphorylation capacity of cryopreserved human cells. Broad adoption of this method will allow uncomplicated collection of samples and measurements of oxidative phosphorylation.

KW - Bioenergetics

KW - Cryopreservation

KW - Immuno-detection

KW - Oxidative phosphorylation

U2 - 10.1016/j.mito.2019.04.011

DO - 10.1016/j.mito.2019.04.011

M3 - Journal article

C2 - 31051261

AN - SCOPUS:85065493789

SN - 1567-7249

VL - 47

SP - 47

EP - 53

JO - Mitochondrion

JF - Mitochondrion

ER -