Microwave-assisted solid-phase peptide synthesis using the biotage syro Wave

Leila Malik, Knud Jørgen Jensen

2 Citationer (Scopus)

Abstract

Fast and precise heating by microwave irradiation during solid-phase peptide synthesis (SPPS) can reduce reaction times as well as provide better purities and greater yields for the synthesis of difficult peptides. Microwave- assisted SPPS has proven to be a useful and reliable tool for the synthesis of peptides as well as small proteins. It is particularly well suited for sequences with a high propensity to form β-sheet-type structures and for sterically difficult couplings. In this protocol, conditions and detailed procedures are described for performing microwave-assisted SPPS using the Syro Wave™. Here we describe the synthesis of two difficult peptide sequences: the first is derived from the C-terminus of the MuLV CTL epitope, the second is a de novo designed peptide with a C-terminal alkyne.

OriginalsprogEngelsk
TitelPeptide synthesis and applications
RedaktørerKnud J. Jensen, Pernille T. Shelton, Søren L. Pedersen
Antal sider10
ForlagHumana Press
Publikationsdato2013
Sider225-234
ISBN (Trykt)978-1-62703-543-9
ISBN (Elektronisk)978-1-62703-544-6
DOI
StatusUdgivet - 2013
NavnMethods in Molecular Biology
Vol/bind1047
ISSN1064-3745

Emneord

  • Backbone amide linker
  • Handle
  • Peptide
  • Aldehyde
  • Thioester
  • N-Alkyl amide
  • Cyclic peptide
  • Reductive amination

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