Maternal loss of miRNAs leads to increased variance in primordial germ cell numbers in Drosophila melanogaster

Jan-Michael Kugler; Ya-Wen Chen; Ruifen Weng; Stephen M Cohen

doi:10.1534/g3.113.007591

Maternal loss of miRNAs leads to increased variance in primordial germ cell numbers in Drosophila melanogaster

Jan-Michael Kugler, Ya-Wen Chen, Ruifen Weng, Stephen M Cohen

18 Citationer (Scopus)

Abstract

MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression that may act as buffering agents to stabilize gene-regulatory networks. Here, we identify two miRNAs that are maternally required for normal embryonic primordial germ cell development in Drosophila melanogaster. Embryos derived from miR-969 and miR-9c mutant mothers had, on average, reduced germ cell numbers. Intriguingly, this reduction correlated with an increase in the variance of this quantitative phenotypic trait. Analysis of an independent set of maternal mutant genotypes suggests that reduction of germ cell number need not lead to increased variance. Our observations are consistent with the hypothesis that miR-969 and miR-9c contribute to stabilizing the processes that control germ number, supporting phenotypic robustness.

Originalsprog	Engelsk
Tidsskrift	G3: Genes, Genomes, Genetics (Bethesda)
Vol/bind	3
Udgave nummer	9
Sider (fra-til)	1573-6
Antal sider	4
ISSN	2160-1836
DOI	https://doi.org/10.1534/g3.113.007591
Status	Udgivet - sep. 2013
Udgivet eksternt	Ja

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10.1534/g3.113.007591

Citationsformater

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title = "Maternal loss of miRNAs leads to increased variance in primordial germ cell numbers in Drosophila melanogaster",

abstract = "MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression that may act as buffering agents to stabilize gene-regulatory networks. Here, we identify two miRNAs that are maternally required for normal embryonic primordial germ cell development in Drosophila melanogaster. Embryos derived from miR-969 and miR-9c mutant mothers had, on average, reduced germ cell numbers. Intriguingly, this reduction correlated with an increase in the variance of this quantitative phenotypic trait. Analysis of an independent set of maternal mutant genotypes suggests that reduction of germ cell number need not lead to increased variance. Our observations are consistent with the hypothesis that miR-969 and miR-9c contribute to stabilizing the processes that control germ number, supporting phenotypic robustness.",

keywords = "Animals, Drosophila melanogaster, Embryo, Nonmammalian, Female, Genotype, Germ Cells, MicroRNAs, Mutation, Phenotype",

author = "Jan-Michael Kugler and Ya-Wen Chen and Ruifen Weng and Cohen, {Stephen M}",

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T1 - Maternal loss of miRNAs leads to increased variance in primordial germ cell numbers in Drosophila melanogaster

AU - Kugler, Jan-Michael

AU - Chen, Ya-Wen

AU - Weng, Ruifen

AU - Cohen, Stephen M

PY - 2013/9

Y1 - 2013/9

N2 - MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression that may act as buffering agents to stabilize gene-regulatory networks. Here, we identify two miRNAs that are maternally required for normal embryonic primordial germ cell development in Drosophila melanogaster. Embryos derived from miR-969 and miR-9c mutant mothers had, on average, reduced germ cell numbers. Intriguingly, this reduction correlated with an increase in the variance of this quantitative phenotypic trait. Analysis of an independent set of maternal mutant genotypes suggests that reduction of germ cell number need not lead to increased variance. Our observations are consistent with the hypothesis that miR-969 and miR-9c contribute to stabilizing the processes that control germ number, supporting phenotypic robustness.

AB - MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression that may act as buffering agents to stabilize gene-regulatory networks. Here, we identify two miRNAs that are maternally required for normal embryonic primordial germ cell development in Drosophila melanogaster. Embryos derived from miR-969 and miR-9c mutant mothers had, on average, reduced germ cell numbers. Intriguingly, this reduction correlated with an increase in the variance of this quantitative phenotypic trait. Analysis of an independent set of maternal mutant genotypes suggests that reduction of germ cell number need not lead to increased variance. Our observations are consistent with the hypothesis that miR-969 and miR-9c contribute to stabilizing the processes that control germ number, supporting phenotypic robustness.

KW - Animals

KW - Drosophila melanogaster

KW - Embryo, Nonmammalian

KW - Female

KW - Genotype

KW - Germ Cells

KW - MicroRNAs

KW - Mutation

KW - Phenotype

U2 - 10.1534/g3.113.007591

DO - 10.1534/g3.113.007591

M3 - Journal article

C2 - 23893743

SN - 2160-1836

VL - 3

SP - 1573

EP - 1576

JO - G3: Genes, Genomes, Genetics (Bethesda)

JF - G3: Genes, Genomes, Genetics (Bethesda)

IS - 9

ER -

Maternal loss of miRNAs leads to increased variance in primordial germ cell numbers in Drosophila melanogaster

Abstract

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