Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy

TY - JOUR

T1 - Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy

AU - Brazhe, Nadezda A

AU - Treiman, Marek

AU - Brazhe, Alexey R

AU - Find, Ninett L

AU - Maksimov, Georgy V

AU - Sosnovtseva, Olga

PY - 2012/9/5

Y1 - 2012/9/5

N2 - This paper presents a nonivasive approach to study redox state of reduced cytochromes c, c1 and b of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy. For the first time with the proposed approach we perform studies of rod- and round-shaped cardiomyocytes, representing different morphological and functional states. Raman mapping and cluster analysis reveal that these cardiomyocytes differ in the amounts of reduced cytochromes c, c1 and b. The rod-shaped cardiomyocytes possess uneven distribution of reduced cytochromes c, c1 and b in cell center and periphery. Moreover, by means of Raman spectroscopy we demonstrated the decrease in the relative amounts of reduced cytochromes c, c1 and b in the rod-shaped cardiomyocytes caused by H2O2-induced oxidative stress before any visible changes. Results of Raman mapping and time-dependent study of reduced cytochromes of complexes II and III and cytochrome c in cardiomyocytes are in a good agreement with our fluorescence indicator studies and other published data.

AB - This paper presents a nonivasive approach to study redox state of reduced cytochromes c, c1 and b of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy. For the first time with the proposed approach we perform studies of rod- and round-shaped cardiomyocytes, representing different morphological and functional states. Raman mapping and cluster analysis reveal that these cardiomyocytes differ in the amounts of reduced cytochromes c, c1 and b. The rod-shaped cardiomyocytes possess uneven distribution of reduced cytochromes c, c1 and b in cell center and periphery. Moreover, by means of Raman spectroscopy we demonstrated the decrease in the relative amounts of reduced cytochromes c, c1 and b in the rod-shaped cardiomyocytes caused by H2O2-induced oxidative stress before any visible changes. Results of Raman mapping and time-dependent study of reduced cytochromes of complexes II and III and cytochrome c in cardiomyocytes are in a good agreement with our fluorescence indicator studies and other published data.

U2 - 10.1371/journal.pone.0041990

DO - 10.1371/journal.pone.0041990

M3 - Journal article

C2 - 22957018

SN - 1932-6203

VL - 7

SP - e41990

JO - PLoS Computational Biology

JF - PLoS Computational Biology

IS - 9

ER -