Malnutrition predisposes to endotoxin-induced edema and impaired inflammatory response in parenterally fed piglets

Ole Bæk; Christian Fabiansen; Henrik Friis; Christian Ritz; Jørgen Koch; Jakob L Willesen; Peter M H Heegaard; Jens Lykkesfeldt; André Briend; Michael H Golden; Per Torp Sangild; Thomas Thymann

doi:10.1002/jpen.1705

Malnutrition predisposes to endotoxin-induced edema and impaired inflammatory response in parenterally fed piglets

Ole Bæk, Christian Fabiansen, Henrik Friis, Christian Ritz, Jørgen Koch, Jakob L Willesen, Peter M H Heegaard, Jens Lykkesfeldt, André Briend, Michael H Golden, Per Torp Sangild, Thomas Thymann^*

^*Corresponding author af dette arbejde

2 Citationer (Scopus)

Abstract

Background: Poor nutrition status is common among hospitalized children and children in low-income countries and may be associated with increased susceptibility to edema and infections. We hypothesized that poor nutrition status, established with a suboptimal composition of parenteral nutrition (PN), predisposes to endotoxemia-induced edema, oxidative stress, and dysregulated immune responses. Methods: Using a 2 × 2 factorial design, 3-day-old piglets (n = 40) were given either optimal or suboptimal composition of PN for 7 days and then infused with either saline or lipopolysaccharide (LPS) for 9 hours to induce an acute-phase reaction. Abdominal tissue edema and blood markers of immunity, inflammation, and oxidative stress were assessed. Results: Piglets receiving suboptimal nutrition showed signs of malnutrition with restricted growth, signs of inflammation (elevated C-reactive protein [CRP], interleukin-6, and serum amyloid A levels), oxidative stress (lower erythrocyte glutathione/hemoglobin and α-tocopherol/cholesterol ratios), and liver dysfunction (increased liver weight and blood bilirubin levels). Perirenal edema was more excessive in malnourished LPS-infused animals, relative to healthy LPS-infused control animals (P <.01). Malnutrition reduced the inflammatory response to LPS (lower CRP, tumor necrosis factor-α, haptoglobin, and neutrophil to lymphocyte ratio) but did not influence LPS-induced oxidative stress markers. Conclusions: We conclude that endotoxemia and malnutrition in combination lead to acute-phase hyporesponsiveness and perirenal edema in piglets. This finding may have implications for pediatric patients that suffer from malnutrition, as their response to bacterial infections may differ substantially from patients of normal nutrition status.

Originalsprog	Engelsk
Tidsskrift	Journal of Parenteral and Enteral Nutrition
ISSN	0148-6071
DOI	https://doi.org/10.1002/jpen.1705
Status	Udgivet - 1 maj 2020

Emneord

Det Natur- og Biovidenskabelige Fakultet

FN’s Verdensmål

Dette resultat bidrager til følgende verdensmål

Adgang til dokumentet

10.1002/jpen.1705

Citationsformater

@article{0158097c80aa4b0aafd5cce320ffb7bd,

title = "Malnutrition predisposes to endotoxin-induced edema and impaired inflammatory response in parenterally fed piglets",

abstract = "Background: Poor nutrition status is common among hospitalized children and children in low-income countries and may be associated with increased susceptibility to edema and infections. We hypothesized that poor nutrition status, established with a suboptimal composition of parenteral nutrition (PN), predisposes to endotoxemia-induced edema, oxidative stress, and dysregulated immune responses. Methods: Using a 2 × 2 factorial design, 3-day-old piglets (n = 40) were given either optimal or suboptimal composition of PN for 7 days and then infused with either saline or lipopolysaccharide (LPS) for 9 hours to induce an acute-phase reaction. Abdominal tissue edema and blood markers of immunity, inflammation, and oxidative stress were assessed. Results: Piglets receiving suboptimal nutrition showed signs of malnutrition with restricted growth, signs of inflammation (elevated C-reactive protein [CRP], interleukin-6, and serum amyloid A levels), oxidative stress (lower erythrocyte glutathione/hemoglobin and α-tocopherol/cholesterol ratios), and liver dysfunction (increased liver weight and blood bilirubin levels). Perirenal edema was more excessive in malnourished LPS-infused animals, relative to healthy LPS-infused control animals (P <.01). Malnutrition reduced the inflammatory response to LPS (lower CRP, tumor necrosis factor-α, haptoglobin, and neutrophil to lymphocyte ratio) but did not influence LPS-induced oxidative stress markers. Conclusions: We conclude that endotoxemia and malnutrition in combination lead to acute-phase hyporesponsiveness and perirenal edema in piglets. This finding may have implications for pediatric patients that suffer from malnutrition, as their response to bacterial infections may differ substantially from patients of normal nutrition status.",

keywords = "Faculty of Science, Edema, Inflammation, Kwashiorkor, Lipopolysaccharide, Malnutrition, Oxidative stress, Piglets",

author = "Ole B{\ae}k and Christian Fabiansen and Henrik Friis and Christian Ritz and J{\o}rgen Koch and Willesen, {Jakob L} and Heegaard, {Peter M H} and Jens Lykkesfeldt and Andr{\'e} Briend and Golden, {Michael H} and Sangild, {Per Torp} and Thomas Thymann",

note = "CURIS 2019 NEXS 298 {\textcopyright} 2019 American Society for Parenteral and Enteral Nutrition.",

year = "2020",

month = may,

day = "1",

doi = "10.1002/jpen.1705",

language = "English",

journal = "Journal of Parenteral and Enteral Nutrition",

issn = "0148-6071",

publisher = "SAGE Publications",

}

TY - JOUR

T1 - Malnutrition predisposes to endotoxin-induced edema and impaired inflammatory response in parenterally fed piglets

AU - Bæk, Ole

AU - Fabiansen, Christian

AU - Friis, Henrik

AU - Ritz, Christian

AU - Koch, Jørgen

AU - Willesen, Jakob L

AU - Heegaard, Peter M H

AU - Lykkesfeldt, Jens

AU - Briend, André

AU - Golden, Michael H

AU - Sangild, Per Torp

AU - Thymann, Thomas

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Background: Poor nutrition status is common among hospitalized children and children in low-income countries and may be associated with increased susceptibility to edema and infections. We hypothesized that poor nutrition status, established with a suboptimal composition of parenteral nutrition (PN), predisposes to endotoxemia-induced edema, oxidative stress, and dysregulated immune responses. Methods: Using a 2 × 2 factorial design, 3-day-old piglets (n = 40) were given either optimal or suboptimal composition of PN for 7 days and then infused with either saline or lipopolysaccharide (LPS) for 9 hours to induce an acute-phase reaction. Abdominal tissue edema and blood markers of immunity, inflammation, and oxidative stress were assessed. Results: Piglets receiving suboptimal nutrition showed signs of malnutrition with restricted growth, signs of inflammation (elevated C-reactive protein [CRP], interleukin-6, and serum amyloid A levels), oxidative stress (lower erythrocyte glutathione/hemoglobin and α-tocopherol/cholesterol ratios), and liver dysfunction (increased liver weight and blood bilirubin levels). Perirenal edema was more excessive in malnourished LPS-infused animals, relative to healthy LPS-infused control animals (P <.01). Malnutrition reduced the inflammatory response to LPS (lower CRP, tumor necrosis factor-α, haptoglobin, and neutrophil to lymphocyte ratio) but did not influence LPS-induced oxidative stress markers. Conclusions: We conclude that endotoxemia and malnutrition in combination lead to acute-phase hyporesponsiveness and perirenal edema in piglets. This finding may have implications for pediatric patients that suffer from malnutrition, as their response to bacterial infections may differ substantially from patients of normal nutrition status.

AB - Background: Poor nutrition status is common among hospitalized children and children in low-income countries and may be associated with increased susceptibility to edema and infections. We hypothesized that poor nutrition status, established with a suboptimal composition of parenteral nutrition (PN), predisposes to endotoxemia-induced edema, oxidative stress, and dysregulated immune responses. Methods: Using a 2 × 2 factorial design, 3-day-old piglets (n = 40) were given either optimal or suboptimal composition of PN for 7 days and then infused with either saline or lipopolysaccharide (LPS) for 9 hours to induce an acute-phase reaction. Abdominal tissue edema and blood markers of immunity, inflammation, and oxidative stress were assessed. Results: Piglets receiving suboptimal nutrition showed signs of malnutrition with restricted growth, signs of inflammation (elevated C-reactive protein [CRP], interleukin-6, and serum amyloid A levels), oxidative stress (lower erythrocyte glutathione/hemoglobin and α-tocopherol/cholesterol ratios), and liver dysfunction (increased liver weight and blood bilirubin levels). Perirenal edema was more excessive in malnourished LPS-infused animals, relative to healthy LPS-infused control animals (P <.01). Malnutrition reduced the inflammatory response to LPS (lower CRP, tumor necrosis factor-α, haptoglobin, and neutrophil to lymphocyte ratio) but did not influence LPS-induced oxidative stress markers. Conclusions: We conclude that endotoxemia and malnutrition in combination lead to acute-phase hyporesponsiveness and perirenal edema in piglets. This finding may have implications for pediatric patients that suffer from malnutrition, as their response to bacterial infections may differ substantially from patients of normal nutrition status.

KW - Faculty of Science

KW - Edema

KW - Inflammation

KW - Kwashiorkor

KW - Lipopolysaccharide

KW - Malnutrition

KW - Oxidative stress

KW - Piglets

U2 - 10.1002/jpen.1705

DO - 10.1002/jpen.1705

M3 - Journal article

C2 - 31512266

SN - 0148-6071

JO - Journal of Parenteral and Enteral Nutrition

JF - Journal of Parenteral and Enteral Nutrition

ER -

Malnutrition predisposes to endotoxin-induced edema and impaired inflammatory response in parenterally fed piglets

Abstract

Emneord

FN’s Verdensmål

Adgang til dokumentet

Fingeraftryk

Citationsformater