Abstract
Treatment in vitro of Ehrlich ascites tumor cells or human fibroblasts with 8-methoxypsoralen (8-MOP, 2.4 microM) and UVA irradiation results in a 30% and 60% respectively reduction in lysosomal beta-galactosidase activity in situ. Under identical conditions one 8-MOP adduct was formed per 2 X 10(4) bases of DNA, one 8-MOP adduct was formed per approximately 10(4) tRNA molecules and one per approximately 100 ribosomes. It is suggested that the decrease in lysosomal beta-galactosidase activity is a result of leakage through the lysosomal membrane caused by psoralen-UVA damage of the lipids in the membrane, since no effect was found on beta-galactosidase in vitro. These results indicate that the lysosomes may also be a target for cellular photodamage by 8-methoxy-psoralen.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of photochemistry and photobiology. B, Biology |
| Vol/bind | 3 |
| Udgave nummer | 3 |
| Sider (fra-til) | 437-47 |
| Antal sider | 11 |
| ISSN | 1011-1344 |
| Status | Udgivet - jun. 1989 |