Latent manganese deficiency increases transpiration in barley (Hordeum vulgare)

Christopher Alan Hebbern; Kristian Holst Laursen; Anne Hald Ladegaard; Sidsel Birkelund Schmidt; Pai Rosager Pedas; Dan Bruhn; Jan Kofod Schjørring; Dvora-Laio Wulfsohn; Søren Husted

doi:10.1111/j.1399-3054.2008.01188.x

Latent manganese deficiency increases transpiration in barley (Hordeum vulgare)

Christopher Alan Hebbern, Kristian Holst Laursen, Anne Hald Ladegaard, Sidsel Birkelund Schmidt, Pai Rosager Pedas, Dan Bruhn, Jan Kofod Schjørring, Dvora-Laio Wulfsohn, Søren Husted

54 Citationer (Scopus)

Abstract

To investigate if latent manganese (Mn) deficiency leads to increased transpiration, barley plants were grown for 10 weeks in hydroponics with daily additions of Mn in the low nM range. The Mn-starved plants did not exhibit visual leaf symptoms of Mn deficiency, but Chl a fluorescence measurements revealed that the quantum yield efficiency of PSII (F_v/F_m) was reduced from 0.83 in Mn-sufficient control plants to below 0.5 in Mn-starved plants. Leaf Mn concentrations declined from 30 to 7 µg Mn g^-1 dry weight in control and Mn-starved plants, respectively. Mn-starved plants had up to four-fold higher transpiration than control plants. Stomatal closure and opening upon light/dark transitions took place at the same rate in both Mn treatments, but the nocturnal leaf conductance for water vapour was still twice as high in Mn-starved plants compared with the control. The observed increase in transpiration was substantiated by ¹³C-isotope discrimination analysis and gravimetric measurement of the water consumption, showing significantly lower water use efficiency in Mn-starved plants. The extractable wax content of leaves of Mn-starved plants was approximately 40% lower than that in control plants, and it is concluded that the increased leaf conductance and higher transpirational water loss are correlated with a reduction in the epicuticular wax layer under Mn deficiency.

Originalsprog	Engelsk
Tidsskrift	Physiologia Plantarum : An International Journal for Plant Biology
Vol/bind	135
Udgave nummer	3
Sider (fra-til)	307-316
Antal sider	10
ISSN	0031-9317
DOI	https://doi.org/10.1111/j.1399-3054.2008.01188.x
Status	Udgivet - 2009

Adgang til dokumentet

10.1111/j.1399-3054.2008.01188.x

Citationsformater

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title = "Latent manganese deficiency increases transpiration in barley (Hordeum vulgare)",

abstract = "To investigate if latent manganese (Mn) deficiency leads to increased transpiration, barley plants were grown for 10 weeks in hydroponics with daily additions of Mn in the low nM range. The Mn-starved plants did not exhibit visual leaf symptoms of Mn deficiency, but Chl a fluorescence measurements revealed that the quantum yield efficiency of PSII (Fv/Fm) was reduced from 0.83 in Mn-sufficient control plants to below 0.5 in Mn-starved plants. Leaf Mn concentrations declined from 30 to 7 µg Mn g-1 dry weight in control and Mn-starved plants, respectively. Mn-starved plants had up to four-fold higher transpiration than control plants. Stomatal closure and opening upon light/dark transitions took place at the same rate in both Mn treatments, but the nocturnal leaf conductance for water vapour was still twice as high in Mn-starved plants compared with the control. The observed increase in transpiration was substantiated by 13C-isotope discrimination analysis and gravimetric measurement of the water consumption, showing significantly lower water use efficiency in Mn-starved plants. The extractable wax content of leaves of Mn-starved plants was approximately 40% lower than that in control plants, and it is concluded that the increased leaf conductance and higher transpirational water loss are correlated with a reduction in the epicuticular wax layer under Mn deficiency.",

author = "Hebbern, {Christopher Alan} and Laursen, {Kristian Holst} and Ladegaard, {Anne Hald} and Schmidt, {Sidsel Birkelund} and Pedas, {Pai Rosager} and Dan Bruhn and Schj{\o}rring, {Jan Kofod} and Dvora-Laio Wulfsohn and S{\o}ren Husted",

year = "2009",

doi = "10.1111/j.1399-3054.2008.01188.x",

language = "English",

volume = "135",

pages = "307--316",

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TY - JOUR

T1 - Latent manganese deficiency increases transpiration in barley (Hordeum vulgare)

AU - Hebbern, Christopher Alan

AU - Laursen, Kristian Holst

AU - Ladegaard, Anne Hald

AU - Schmidt, Sidsel Birkelund

AU - Pedas, Pai Rosager

AU - Bruhn, Dan

AU - Schjørring, Jan Kofod

AU - Wulfsohn, Dvora-Laio

AU - Husted, Søren

PY - 2009

Y1 - 2009

N2 - To investigate if latent manganese (Mn) deficiency leads to increased transpiration, barley plants were grown for 10 weeks in hydroponics with daily additions of Mn in the low nM range. The Mn-starved plants did not exhibit visual leaf symptoms of Mn deficiency, but Chl a fluorescence measurements revealed that the quantum yield efficiency of PSII (Fv/Fm) was reduced from 0.83 in Mn-sufficient control plants to below 0.5 in Mn-starved plants. Leaf Mn concentrations declined from 30 to 7 µg Mn g-1 dry weight in control and Mn-starved plants, respectively. Mn-starved plants had up to four-fold higher transpiration than control plants. Stomatal closure and opening upon light/dark transitions took place at the same rate in both Mn treatments, but the nocturnal leaf conductance for water vapour was still twice as high in Mn-starved plants compared with the control. The observed increase in transpiration was substantiated by 13C-isotope discrimination analysis and gravimetric measurement of the water consumption, showing significantly lower water use efficiency in Mn-starved plants. The extractable wax content of leaves of Mn-starved plants was approximately 40% lower than that in control plants, and it is concluded that the increased leaf conductance and higher transpirational water loss are correlated with a reduction in the epicuticular wax layer under Mn deficiency.

AB - To investigate if latent manganese (Mn) deficiency leads to increased transpiration, barley plants were grown for 10 weeks in hydroponics with daily additions of Mn in the low nM range. The Mn-starved plants did not exhibit visual leaf symptoms of Mn deficiency, but Chl a fluorescence measurements revealed that the quantum yield efficiency of PSII (Fv/Fm) was reduced from 0.83 in Mn-sufficient control plants to below 0.5 in Mn-starved plants. Leaf Mn concentrations declined from 30 to 7 µg Mn g-1 dry weight in control and Mn-starved plants, respectively. Mn-starved plants had up to four-fold higher transpiration than control plants. Stomatal closure and opening upon light/dark transitions took place at the same rate in both Mn treatments, but the nocturnal leaf conductance for water vapour was still twice as high in Mn-starved plants compared with the control. The observed increase in transpiration was substantiated by 13C-isotope discrimination analysis and gravimetric measurement of the water consumption, showing significantly lower water use efficiency in Mn-starved plants. The extractable wax content of leaves of Mn-starved plants was approximately 40% lower than that in control plants, and it is concluded that the increased leaf conductance and higher transpirational water loss are correlated with a reduction in the epicuticular wax layer under Mn deficiency.

U2 - 10.1111/j.1399-3054.2008.01188.x

DO - 10.1111/j.1399-3054.2008.01188.x

M3 - Journal article

C2 - 19140891

SN - 0031-9317

VL - 135

SP - 307

EP - 316

JO - Physiologia Plantarum

JF - Physiologia Plantarum

IS - 3

ER -