Laparoscopic Roux-en-Y gastric bypass in super obese Göttingen minipigs

TY - JOUR

T1 - Laparoscopic Roux-en-Y gastric bypass in super obese Göttingen minipigs

AU - Birck, Malene Muusfeldt

AU - Vegge, Andreas

AU - Støckel, Mikael

AU - Gögenür, Ismayil

AU - Thymann, Thomas

AU - Hammelev, Karsten Pharao

AU - Sangild, Per Torp

AU - Hansen, Axel Jacob Kornerup

AU - Raun, Kirsten

AU - von Voss, Pia

AU - Eriksen, Thomas

N1 - CURIS 2013 NEXS 313

PY - 2013

Y1 - 2013

N2 - Background: The specific mechanisms behind weight loss and comorbidity improvements in obese patients after Roux-en-Y gastric bypass (RYGBP) are still poorly understood. The aim of this study was to establish and evaluate the feasibility of a long-term survival RYGBP model in super obese Göttingen minipigs in order to improve the translational potential relative to current animal models. Methods: Eleven Göttingen minipigs with diet-induced obesity underwent laparoscopic RYGBP and were followed up to 9 months after surgery. Intra-and post-operative complications, body weight (BW), food intake and necropsy data were recorded. Results: Five minipigs survived without complications to the end of the study. Four minipigs developed surgical related complications and were euthanized while two minipigs died due to central venous catheter related complications. BW and food intake is reported for the six minipigs surviving longer than 4.5 months post-surgery. Weight loss and reduced food intake was seen in all minipigs. After 2-3 months of weight loss, weight regain was evident in all but two minipigs which seemed to continue losing weight. Necropsy revealed some variation in the length of the alimentary, biliary and common limb between minipigs. Conclusion: The use of obese Göttingen minipigs as a translational RYGBP model is feasible and has potential for the study of RYGBP-related changes in gut function, type-2 diabetes and appetite regulation. Still, the surgical procedure is technically highly demanding in obese Göttingen minipigs and the peri-operative animal care and follow up requires close monitoring.

AB - Background: The specific mechanisms behind weight loss and comorbidity improvements in obese patients after Roux-en-Y gastric bypass (RYGBP) are still poorly understood. The aim of this study was to establish and evaluate the feasibility of a long-term survival RYGBP model in super obese Göttingen minipigs in order to improve the translational potential relative to current animal models. Methods: Eleven Göttingen minipigs with diet-induced obesity underwent laparoscopic RYGBP and were followed up to 9 months after surgery. Intra-and post-operative complications, body weight (BW), food intake and necropsy data were recorded. Results: Five minipigs survived without complications to the end of the study. Four minipigs developed surgical related complications and were euthanized while two minipigs died due to central venous catheter related complications. BW and food intake is reported for the six minipigs surviving longer than 4.5 months post-surgery. Weight loss and reduced food intake was seen in all minipigs. After 2-3 months of weight loss, weight regain was evident in all but two minipigs which seemed to continue losing weight. Necropsy revealed some variation in the length of the alimentary, biliary and common limb between minipigs. Conclusion: The use of obese Göttingen minipigs as a translational RYGBP model is feasible and has potential for the study of RYGBP-related changes in gut function, type-2 diabetes and appetite regulation. Still, the surgical procedure is technically highly demanding in obese Göttingen minipigs and the peri-operative animal care and follow up requires close monitoring.

KW - Faculty of Health and Medical Sciences

KW - Gastric bypass

KW - obesity

KW - diabetes

KW - weight loss

KW - animal model

KW - pig

UR - http://www.scopus.com/inward/record.url?scp=84885080063&partnerID=8YFLogxK

M3 - Journal article

C2 - 24093061

AN - SCOPUS:84885080063

SN - 1943-8141

VL - 5

SP - 643

EP - 653

JO - American Journal of Translational Research

JF - American Journal of Translational Research

IS - 6

ER -