TY - JOUR
T1 - Knotty Problems during Mitosis
T2 - Mechanistic Insight into the Processing of Ultrafine DNA Bridges in Anaphase
AU - Sarlós, Kata
AU - Biebricher, Andreas
AU - Petermann, Erwin J G
AU - Wuite, Gijs J L
AU - Hickson, Ian D
N1 - © 2017 Sarlós et al.; Published by Cold Spring Harbor Laboratory Press.
PY - 2017
Y1 - 2017
N2 - To survive and proliferate, cells have to faithfully segregate their newly replicated genomic DNA to the two daughter cells. However, the sister chromatids of mitotic chromosomes are frequently interlinked by so-called ultrafine DNA bridges (UFBs) that are visible in the anaphase of mitosis. UFBs can only be detected by the proteins bound to them and not by staining with conventional DNA dyes. These DNA bridges are presumed to represent entangled sister chromatids and hence pose a threat to faithful segregation. A failure to accurately unlink UFB DNA results in chromosome segregation errors and binucleation. This, in turn, compromises genome integrity, which is a hallmark of cancer. UFBs are actively removed during anaphase, and most known UFB-associated proteins are enzymes involved in DNA repair in interphase. However, little is known about the mitotic activities of these enzymes or the exact DNA structures present on UFBs. We focus on the biology of UFBs, with special emphasis on their underlying DNA structure and the decatenation machineries that process UFBs.
AB - To survive and proliferate, cells have to faithfully segregate their newly replicated genomic DNA to the two daughter cells. However, the sister chromatids of mitotic chromosomes are frequently interlinked by so-called ultrafine DNA bridges (UFBs) that are visible in the anaphase of mitosis. UFBs can only be detected by the proteins bound to them and not by staining with conventional DNA dyes. These DNA bridges are presumed to represent entangled sister chromatids and hence pose a threat to faithful segregation. A failure to accurately unlink UFB DNA results in chromosome segregation errors and binucleation. This, in turn, compromises genome integrity, which is a hallmark of cancer. UFBs are actively removed during anaphase, and most known UFB-associated proteins are enzymes involved in DNA repair in interphase. However, little is known about the mitotic activities of these enzymes or the exact DNA structures present on UFBs. We focus on the biology of UFBs, with special emphasis on their underlying DNA structure and the decatenation machineries that process UFBs.
U2 - 10.1101/sqb.2017.82.033647
DO - 10.1101/sqb.2017.82.033647
M3 - Journal article
C2 - 29167280
SN - 0091-7451
VL - 82
SP - 187
EP - 195
JO - Cold Spring Harbor Laboratory. Symposia on Quantitative Biology
JF - Cold Spring Harbor Laboratory. Symposia on Quantitative Biology
ER -
