Investigation of the charge effect on the electrochemical transduction in a quinone-based DNA sensor

TY - JOUR

T1 - Investigation of the charge effect on the electrochemical transduction in a quinone-based DNA sensor

AU - Reisberg, S.

AU - Piro, B.

AU - Noel, V.

AU - Nguyen, T.D.

AU - Nielsen, Peter E.

AU - Pham, M.C.

PY - 2008

Y1 - 2008

N2 - To elucidate the mechanism involved in the electrochemical transduction process of a conducting polymer-based DNA sensor, peptide nucleic acids (PNA) were used. PNA are DNA analogues having similar hybridization properties but are neutral. This allows to discriminate the electrostatic effect of DNA strands from the steric hindrance generated on the bioelectrode upon hybridization. It can be concluded that DNA conformational changes are determinant in the transduction process and that the electrostatic effect is negligible.

AB - To elucidate the mechanism involved in the electrochemical transduction process of a conducting polymer-based DNA sensor, peptide nucleic acids (PNA) were used. PNA are DNA analogues having similar hybridization properties but are neutral. This allows to discriminate the electrostatic effect of DNA strands from the steric hindrance generated on the bioelectrode upon hybridization. It can be concluded that DNA conformational changes are determinant in the transduction process and that the electrostatic effect is negligible.

M3 - Journal article

SN - 0013-4686

VL - 54

SP - 346

EP - 351

JO - Electrochimica Acta

JF - Electrochimica Acta

IS - 2

ER -