Integration of waveguides for optical detection in microfabricated analytical devices

TY - GEN

T1 - Integration of waveguides for optical detection in microfabricated analytical devices

AU - Kutter, Jorg P.

AU - Mogensen, Klaus B.

AU - Friis, Peter

AU - Jorgensen, Anders M.

AU - Petersen, Nickolaj J.

AU - Telleman, Pieter

AU - Hubner, Jorg

PY - 2000/12/1

Y1 - 2000/12/1

N2 - Buried optical channel waveguides integrated with a fluidic channel network on a planar microdevice are presented. The waveguides were fabricated using silica-on-silicon technology with the goal to replace bulk optical elements and facilitate various optical detection techniques for miniaturized total analysis systems or lab-on-a-chip systems. Waveguide structures with core layers doped with germanium were employed for fluorescence measurements, while waveguides with nitrogen-only doped core layers were used for absorbance measurements. By the elimination of germanium oxygen deficiency centers transmission of light down to 210 nm was possible, allowing absorbance measurements in the mid and far UV region (210 to 280 nm), which is the region where a large number of different molecules absorb light. Robust, alignment-free microdevices, which can easily be hooked up to a number of light sources and detectors, were used for fluorescence measurements of two dyes, fluorescein and Bodipy, and absorbance measurements of a stress-reducing drug, propranolol. The lowest detected concentrations were 250 pM for fluorescein, 100 nM for Bodipy and 12 μM for propranolol.

AB - Buried optical channel waveguides integrated with a fluidic channel network on a planar microdevice are presented. The waveguides were fabricated using silica-on-silicon technology with the goal to replace bulk optical elements and facilitate various optical detection techniques for miniaturized total analysis systems or lab-on-a-chip systems. Waveguide structures with core layers doped with germanium were employed for fluorescence measurements, while waveguides with nitrogen-only doped core layers were used for absorbance measurements. By the elimination of germanium oxygen deficiency centers transmission of light down to 210 nm was possible, allowing absorbance measurements in the mid and far UV region (210 to 280 nm), which is the region where a large number of different molecules absorb light. Robust, alignment-free microdevices, which can easily be hooked up to a number of light sources and detectors, were used for fluorescence measurements of two dyes, fluorescein and Bodipy, and absorbance measurements of a stress-reducing drug, propranolol. The lowest detected concentrations were 250 pM for fluorescein, 100 nM for Bodipy and 12 μM for propranolol.

UR - http://www.scopus.com/inward/record.url?scp=0034547211&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:0034547211

SN - 0277-786X

VL - 4177

SP - 98

EP - 105

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Microfluidic Devices and Systems III

Y2 - 18 September 2000 through 19 September 2000

ER -