Infectious potential of human derived uropathogenic Escherichia coli UTI86 in the reproductive tract of laying hens

Sisse Mortensen; Andreas Eske Johansen; Ida Thøfner; Jens Peter Christensen; Susanne Elisabeth Pors; Ana Herrero Fresno; Jakob Møller-Jensen; John Elmerdahl Olsen

doi:10.1016/j.vetmic.2019.108445

Infectious potential of human derived uropathogenic Escherichia coli UTI86 in the reproductive tract of laying hens

Sisse Mortensen, Andreas Eske Johansen, Ida Thøfner, Jens Peter Christensen, Susanne Elisabeth Pors, Ana Herrero Fresno, Jakob Møller-Jensen, John Elmerdahl Olsen^*

^*Corresponding author af dette arbejde

3 Citationer (Scopus)

Abstract

Avian pathogenic E. coli (APEC) and human uropathogenic E. coli (UPEC) harbour common virulence factors in spite of being associated with disease in different hosts. APEC strains have been shown to have zoonotic potential. In contrast, it is not known whether UPEC strains can cause infection in immunologically competent hens. The objective of the current study was to compare the ability of the well-characterized UPEC strain, UTI89, and the APEC strain, F149H1S2, to infect human and avian cells in culture and to cause salpingitis in an infection model in adult laying hens. In vitro characterization showed that the strains grew equally well in human urine, and both were able to infect human intestinal (Int407) and bladder (J82) epithelial cell lines, and they survived in avian macrophages (HD11) to the same extent. Groups of adult birds were inoculated with 10⁸ bacteria directly into the oviduct using a surgical procedure. After an infection period of 48 h, bacterial load in the oviduct was determined by dilution series, and pathology was determined based on gross lesions and histological observations. Similar counts of UPEC UTI89 (ST95) and the APEC strain F149H1S2 (ST117) were obtained from tissues of infected birds, and salpingitis as evaluated by clinical score and histopathology was observed to a similar extent after infection with the two strains. Together, the results showed that UPEC UTI89 and APEC F149H1S2 have a similar potential for causing salpingitis in laying hens in the model used. No infection differences were observed between the UPEC UTI89 wild type and a mutant strain with knock-out of the well-known virulence gene, fimH, (UPEC UTI89ΔfimH), showing that the salpingitis model is not suitable for the detection of all UPEC virulence factors.

Originalsprog	Engelsk
Artikelnummer	108445
Tidsskrift	Veterinary Microbiology
Vol/bind	239
ISSN	0378-1135
DOI	https://doi.org/10.1016/j.vetmic.2019.108445
Status	Udgivet - 2019

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@article{834f33831c30469d9e4e06ff6ba28950,

title = "Infectious potential of human derived uropathogenic Escherichia coli UTI86 in the reproductive tract of laying hens",

abstract = "Avian pathogenic E. coli (APEC) and human uropathogenic E. coli (UPEC) harbour common virulence factors in spite of being associated with disease in different hosts. APEC strains have been shown to have zoonotic potential. In contrast, it is not known whether UPEC strains can cause infection in immunologically competent hens. The objective of the current study was to compare the ability of the well-characterized UPEC strain, UTI89, and the APEC strain, F149H1S2, to infect human and avian cells in culture and to cause salpingitis in an infection model in adult laying hens. In vitro characterization showed that the strains grew equally well in human urine, and both were able to infect human intestinal (Int407) and bladder (J82) epithelial cell lines, and they survived in avian macrophages (HD11) to the same extent. Groups of adult birds were inoculated with 108 bacteria directly into the oviduct using a surgical procedure. After an infection period of 48 h, bacterial load in the oviduct was determined by dilution series, and pathology was determined based on gross lesions and histological observations. Similar counts of UPEC UTI89 (ST95) and the APEC strain F149H1S2 (ST117) were obtained from tissues of infected birds, and salpingitis as evaluated by clinical score and histopathology was observed to a similar extent after infection with the two strains. Together, the results showed that UPEC UTI89 and APEC F149H1S2 have a similar potential for causing salpingitis in laying hens in the model used. No infection differences were observed between the UPEC UTI89 wild type and a mutant strain with knock-out of the well-known virulence gene, fimH, (UPEC UTI89ΔfimH), showing that the salpingitis model is not suitable for the detection of all UPEC virulence factors.",

keywords = "Anthrozoonosis, Avian pathogenic E. coli, Salpingitis, Urinary tract infection, Uropathogenic E. coli",

author = "Sisse Mortensen and Johansen, {Andreas Eske} and Ida Th{\o}fner and Christensen, {Jens Peter} and Pors, {Susanne Elisabeth} and Fresno, {Ana Herrero} and Jakob M{\o}ller-Jensen and Olsen, {John Elmerdahl}",

year = "2019",

doi = "10.1016/j.vetmic.2019.108445",

language = "English",

volume = "239",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier",

}

TY - JOUR

T1 - Infectious potential of human derived uropathogenic Escherichia coli UTI86 in the reproductive tract of laying hens

AU - Mortensen, Sisse

AU - Johansen, Andreas Eske

AU - Thøfner, Ida

AU - Christensen, Jens Peter

AU - Pors, Susanne Elisabeth

AU - Fresno, Ana Herrero

AU - Møller-Jensen, Jakob

AU - Olsen, John Elmerdahl

PY - 2019

Y1 - 2019

N2 - Avian pathogenic E. coli (APEC) and human uropathogenic E. coli (UPEC) harbour common virulence factors in spite of being associated with disease in different hosts. APEC strains have been shown to have zoonotic potential. In contrast, it is not known whether UPEC strains can cause infection in immunologically competent hens. The objective of the current study was to compare the ability of the well-characterized UPEC strain, UTI89, and the APEC strain, F149H1S2, to infect human and avian cells in culture and to cause salpingitis in an infection model in adult laying hens. In vitro characterization showed that the strains grew equally well in human urine, and both were able to infect human intestinal (Int407) and bladder (J82) epithelial cell lines, and they survived in avian macrophages (HD11) to the same extent. Groups of adult birds were inoculated with 108 bacteria directly into the oviduct using a surgical procedure. After an infection period of 48 h, bacterial load in the oviduct was determined by dilution series, and pathology was determined based on gross lesions and histological observations. Similar counts of UPEC UTI89 (ST95) and the APEC strain F149H1S2 (ST117) were obtained from tissues of infected birds, and salpingitis as evaluated by clinical score and histopathology was observed to a similar extent after infection with the two strains. Together, the results showed that UPEC UTI89 and APEC F149H1S2 have a similar potential for causing salpingitis in laying hens in the model used. No infection differences were observed between the UPEC UTI89 wild type and a mutant strain with knock-out of the well-known virulence gene, fimH, (UPEC UTI89ΔfimH), showing that the salpingitis model is not suitable for the detection of all UPEC virulence factors.

AB - Avian pathogenic E. coli (APEC) and human uropathogenic E. coli (UPEC) harbour common virulence factors in spite of being associated with disease in different hosts. APEC strains have been shown to have zoonotic potential. In contrast, it is not known whether UPEC strains can cause infection in immunologically competent hens. The objective of the current study was to compare the ability of the well-characterized UPEC strain, UTI89, and the APEC strain, F149H1S2, to infect human and avian cells in culture and to cause salpingitis in an infection model in adult laying hens. In vitro characterization showed that the strains grew equally well in human urine, and both were able to infect human intestinal (Int407) and bladder (J82) epithelial cell lines, and they survived in avian macrophages (HD11) to the same extent. Groups of adult birds were inoculated with 108 bacteria directly into the oviduct using a surgical procedure. After an infection period of 48 h, bacterial load in the oviduct was determined by dilution series, and pathology was determined based on gross lesions and histological observations. Similar counts of UPEC UTI89 (ST95) and the APEC strain F149H1S2 (ST117) were obtained from tissues of infected birds, and salpingitis as evaluated by clinical score and histopathology was observed to a similar extent after infection with the two strains. Together, the results showed that UPEC UTI89 and APEC F149H1S2 have a similar potential for causing salpingitis in laying hens in the model used. No infection differences were observed between the UPEC UTI89 wild type and a mutant strain with knock-out of the well-known virulence gene, fimH, (UPEC UTI89ΔfimH), showing that the salpingitis model is not suitable for the detection of all UPEC virulence factors.

KW - Anthrozoonosis

KW - Avian pathogenic E. coli

KW - Salpingitis

KW - Urinary tract infection

KW - Uropathogenic E. coli

U2 - 10.1016/j.vetmic.2019.108445

DO - 10.1016/j.vetmic.2019.108445

M3 - Journal article

C2 - 31767071

AN - SCOPUS:85073416345

SN - 0378-1135

VL - 239

JO - Veterinary Microbiology

JF - Veterinary Microbiology

M1 - 108445

ER -

Infectious potential of human derived uropathogenic Escherichia coli UTI86 in the reproductive tract of laying hens

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