TY - JOUR
T1 - Improved quantification of stress proteins by western blotting
AU - Lundebye, Anne-Katrine
AU - R. Vedel, Grace
AU - Christensen, Anne M. Krogsdam
AU - Kristiansen, Karsten
AU - Hunter, Douglas
AU - Depledge, Michael H.
N1 - Western blotting; Stress proteins; Proteins; Carcinus maenas; Mytilus edulis
PY - 1995
Y1 - 1995
N2 - An improved method for the semi-quantification of stress protein biomarkers by western blotting is described. The construction of a calibration curve on each gel (using a standard) to calculate relative stress protein levels improved reproducibility of results both within and among blots. Variability in relative stress protein levels of replicate samples run on separate gels was reduced from ca. 24% to 13% when related to a calibration curve rather than being based on direct comparison of absorbance units. Within blot variability was reduced from 5.2-11.7% to 3.4-6.8% by calibrating stress-70 levels. The potential significance of this methodological development is discussed with regard to the literature.
AB - An improved method for the semi-quantification of stress protein biomarkers by western blotting is described. The construction of a calibration curve on each gel (using a standard) to calculate relative stress protein levels improved reproducibility of results both within and among blots. Variability in relative stress protein levels of replicate samples run on separate gels was reduced from ca. 24% to 13% when related to a calibration curve rather than being based on direct comparison of absorbance units. Within blot variability was reduced from 5.2-11.7% to 3.4-6.8% by calibrating stress-70 levels. The potential significance of this methodological development is discussed with regard to the literature.
U2 - 10.1016/0003-2670(95)00162-S
DO - 10.1016/0003-2670(95)00162-S
M3 - Journal article
SN - 0003-2670
VL - 311
SP - 109
EP - 114
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1
ER -
