Improved quantification of stress proteins by western blotting

Anne-Katrine Lundebye; Grace R. Vedel; Anne M. Krogsdam Christensen; Karsten Kristiansen; Douglas Hunter; Michael H. Depledge

doi:10.1016/0003-2670(95)00162-S

Improved quantification of stress proteins by western blotting

Anne-Katrine Lundebye, Grace R. Vedel, Anne M. Krogsdam Christensen, Karsten Kristiansen, Douglas Hunter, Michael H. Depledge

13 Citations (Scopus)

Abstract

An improved method for the semi-quantification of stress protein biomarkers by western blotting is described. The construction of a calibration curve on each gel (using a standard) to calculate relative stress protein levels improved reproducibility of results both within and among blots. Variability in relative stress protein levels of replicate samples run on separate gels was reduced from ca. 24% to 13% when related to a calibration curve rather than being based on direct comparison of absorbance units. Within blot variability was reduced from 5.2-11.7% to 3.4-6.8% by calibrating stress-70 levels. The potential significance of this methodological development is discussed with regard to the literature.

Original language	English
Journal	Analytica Chimica Acta
Volume	311
Issue number	1
Pages (from-to)	109-114
ISSN	0003-2670
DOIs	https://doi.org/10.1016/0003-2670(95)00162-S
Publication status	Published - 1995
Externally published	Yes

Access to Document

10.1016/0003-2670(95)00162-S

Cite this

@article{b47dfd700f1111de8478000ea68e967b,

title = "Improved quantification of stress proteins by western blotting",

abstract = "An improved method for the semi-quantification of stress protein biomarkers by western blotting is described. The construction of a calibration curve on each gel (using a standard) to calculate relative stress protein levels improved reproducibility of results both within and among blots. Variability in relative stress protein levels of replicate samples run on separate gels was reduced from ca. 24% to 13% when related to a calibration curve rather than being based on direct comparison of absorbance units. Within blot variability was reduced from 5.2-11.7% to 3.4-6.8% by calibrating stress-70 levels. The potential significance of this methodological development is discussed with regard to the literature.",

author = "Anne-Katrine Lundebye and {R. Vedel}, Grace and Christensen, {Anne M. Krogsdam} and Karsten Kristiansen and Douglas Hunter and Depledge, {Michael H.}",

note = "Western blotting; Stress proteins; Proteins; Carcinus maenas; Mytilus edulis",

year = "1995",

doi = "10.1016/0003-2670(95)00162-S",

language = "English",

volume = "311",

pages = "109--114",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Improved quantification of stress proteins by western blotting

AU - Lundebye, Anne-Katrine

AU - R. Vedel, Grace

AU - Christensen, Anne M. Krogsdam

AU - Kristiansen, Karsten

AU - Hunter, Douglas

AU - Depledge, Michael H.

N1 - Western blotting; Stress proteins; Proteins; Carcinus maenas; Mytilus edulis

PY - 1995

Y1 - 1995

N2 - An improved method for the semi-quantification of stress protein biomarkers by western blotting is described. The construction of a calibration curve on each gel (using a standard) to calculate relative stress protein levels improved reproducibility of results both within and among blots. Variability in relative stress protein levels of replicate samples run on separate gels was reduced from ca. 24% to 13% when related to a calibration curve rather than being based on direct comparison of absorbance units. Within blot variability was reduced from 5.2-11.7% to 3.4-6.8% by calibrating stress-70 levels. The potential significance of this methodological development is discussed with regard to the literature.

AB - An improved method for the semi-quantification of stress protein biomarkers by western blotting is described. The construction of a calibration curve on each gel (using a standard) to calculate relative stress protein levels improved reproducibility of results both within and among blots. Variability in relative stress protein levels of replicate samples run on separate gels was reduced from ca. 24% to 13% when related to a calibration curve rather than being based on direct comparison of absorbance units. Within blot variability was reduced from 5.2-11.7% to 3.4-6.8% by calibrating stress-70 levels. The potential significance of this methodological development is discussed with regard to the literature.

U2 - 10.1016/0003-2670(95)00162-S

DO - 10.1016/0003-2670(95)00162-S

M3 - Journal article

SN - 0003-2670

VL - 311

SP - 109

EP - 114

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1

ER -

Improved quantification of stress proteins by western blotting

Abstract

Access to Document

Fingerprint

Cite this