Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry

TY - JOUR

T1 - Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry

AU - Kuhnert, Peter

AU - Bisgaard, Magne

AU - Korczak, Bozena M.

AU - Schwendener, Sybille

AU - Christensen, Henrik

AU - Frey, Joachim

PY - 2012/4

Y1 - 2012/4

N2 - Species of the family Pasteurellaceae play an important role as primary or opportunistic animal pathogens. In veterinary diagnostic laboratories identification of this group of bacteria is mainly done by phenotypic assays while genetic identification based on housekeeping genes is mostly used for research and particularly important diagnostic samples. MALDI-TOF MS seems to represent a promising alternative to the currently practiced cumbersome, phenotypic diagnostics carried out in many veterinary diagnostic laboratories. We therefore assessed its application for animal associated members of the family Pasteurellaceae. The Bruker Biotyper 3.0 database was complemented with reference spectra of clinically relevant as well as commensal animal Pasteurellaceae species using generally five strains per species or subspecies and tested for its diagnostic potential with additional, well characterized field isolates. About 250 strains comprising 15 genera and more than 40 species and subspecies were included in the study, covering most representatives of the family. A high discrimination at the genus and species level was observed. Problematic discrimination was only observed with some closely related species and subspecies. MALDI-TOF MS was shown to represent a highly potent method for the diagnosis of this group of animal pathogens, combining speed, precision and low running costs.

AB - Species of the family Pasteurellaceae play an important role as primary or opportunistic animal pathogens. In veterinary diagnostic laboratories identification of this group of bacteria is mainly done by phenotypic assays while genetic identification based on housekeeping genes is mostly used for research and particularly important diagnostic samples. MALDI-TOF MS seems to represent a promising alternative to the currently practiced cumbersome, phenotypic diagnostics carried out in many veterinary diagnostic laboratories. We therefore assessed its application for animal associated members of the family Pasteurellaceae. The Bruker Biotyper 3.0 database was complemented with reference spectra of clinically relevant as well as commensal animal Pasteurellaceae species using generally five strains per species or subspecies and tested for its diagnostic potential with additional, well characterized field isolates. About 250 strains comprising 15 genera and more than 40 species and subspecies were included in the study, covering most representatives of the family. A high discrimination at the genus and species level was observed. Problematic discrimination was only observed with some closely related species and subspecies. MALDI-TOF MS was shown to represent a highly potent method for the diagnosis of this group of animal pathogens, combining speed, precision and low running costs.

KW - Former LIFE faculty

KW - MALDI-TOF MS

KW - Diagnosis

KW - Veterinary medicine

KW - Molecular identifications

KW - Pasteurellaceae

U2 - 10.1016/j.mimet.2012.02.001

DO - 10.1016/j.mimet.2012.02.001

M3 - Journal article

C2 - 22343217

SN - 0167-7012

VL - 89

SP - 1

EP - 7

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -