TY - JOUR
T1 - Human granulosa cells function as innate immune cells executing an inflammatory reaction during ovulation
T2 - a microarray analysis
AU - Poulsen, Liv la Cour
AU - Englund, Anne Lis Mikkelsen
AU - Wissing, Marie Louise Muff
AU - Yding Andersen, Claus
AU - Borup, Rehannah
AU - Grøndahl, Marie Louise
PY - 2019
Y1 - 2019
N2 - Ovulation has been compared to a local inflammatory reaction. We performed an in silico study on a unique, PCR validated, transcriptome microarray study to evaluate if known inflammatory mechanisms operate during ovulation. The granulosa cells were obtained in paired samples at two different time points during ovulation (just before and 36 hours after ovulation induction) from nine women receiving fertility treatment. A total of 259 genes related to inflammation became significantly upregulated during ovulation (2–80 fold, p<0.05), while specific leukocyte markers were absent. The genes and pathway analysis indicated NF-KB-, MAPK- and JAK/STAT signalling (p<1.0E-10) as the major pathways involved in danger recognition and cytokine signalling to initiate inflammation. Upregulated genes further encoded enzymes in eicosanoid production, chemo-attractants, coagulation factors, cell proliferation factors involved in tissue repair, and anti-inflammatory factors to resolve the inflammation again. We conclude that granulosa cells, without involvement from the innate immune system, can orchestrate ovulation as a complete sterile inflammatory reaction.
AB - Ovulation has been compared to a local inflammatory reaction. We performed an in silico study on a unique, PCR validated, transcriptome microarray study to evaluate if known inflammatory mechanisms operate during ovulation. The granulosa cells were obtained in paired samples at two different time points during ovulation (just before and 36 hours after ovulation induction) from nine women receiving fertility treatment. A total of 259 genes related to inflammation became significantly upregulated during ovulation (2–80 fold, p<0.05), while specific leukocyte markers were absent. The genes and pathway analysis indicated NF-KB-, MAPK- and JAK/STAT signalling (p<1.0E-10) as the major pathways involved in danger recognition and cytokine signalling to initiate inflammation. Upregulated genes further encoded enzymes in eicosanoid production, chemo-attractants, coagulation factors, cell proliferation factors involved in tissue repair, and anti-inflammatory factors to resolve the inflammation again. We conclude that granulosa cells, without involvement from the innate immune system, can orchestrate ovulation as a complete sterile inflammatory reaction.
KW - Granulosa cells
KW - Inflammatory response
KW - Innate immunity
KW - LH
KW - Microarray
KW - Ovulation
U2 - 10.1016/j.mce.2019.02.014
DO - 10.1016/j.mce.2019.02.014
M3 - Journal article
C2 - 30802528
AN - SCOPUS:85062300678
SN - 0303-7207
VL - 486
SP - 34
EP - 46
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
ER -