Human embryonic stem cells have enhanced repair of multiple forms of DNA damage

TY - JOUR

T1 - Human embryonic stem cells have enhanced repair of multiple forms of DNA damage

AU - Maynard, Scott

AU - Swistowska, Anna Maria

AU - Lee, Jae Wan

AU - Liu, Ying

AU - Liu, Su-Ting

AU - Da Cruz, Alexandre Bettencourt

AU - Rao, Mahendra

AU - de Souza-Pinto, Nadja C

AU - Zeng, Xianmin

AU - Bohr, Vilhelm A

PY - 2008/9/1

Y1 - 2008/9/1

N2 - Embryonic stem cells need to maintain genomic integrity so that they can retain the ability to differentiate into multiple cell types without propagating DNA errors. Previous studies have suggested that mechanisms of genome surveillance, including DNA repair, are superior in mouse embryonic stem cells compared with various differentiated murine cells. Using single-cell gel electrophoresis (comet assay) we found that human embryonic stem cells (BG01, I6) have more efficient repair of different types of DNA damage (generated from H2O2, UV-C, ionizing radiation, or psoralen) than human primary fibroblasts (WI-38, hs27) and, with the exception of UV-C damage, HeLa cells. Microarray gene expression analysis showed that mRNA levels of several DNA repair genes are elevated in human embryonic stem cells compared with their differentiated forms (embryoid bodies). These data suggest that genomic maintenance pathways are enhanced in human embryonic stem cells, relative to differentiated human cells.

AB - Embryonic stem cells need to maintain genomic integrity so that they can retain the ability to differentiate into multiple cell types without propagating DNA errors. Previous studies have suggested that mechanisms of genome surveillance, including DNA repair, are superior in mouse embryonic stem cells compared with various differentiated murine cells. Using single-cell gel electrophoresis (comet assay) we found that human embryonic stem cells (BG01, I6) have more efficient repair of different types of DNA damage (generated from H2O2, UV-C, ionizing radiation, or psoralen) than human primary fibroblasts (WI-38, hs27) and, with the exception of UV-C damage, HeLa cells. Microarray gene expression analysis showed that mRNA levels of several DNA repair genes are elevated in human embryonic stem cells compared with their differentiated forms (embryoid bodies). These data suggest that genomic maintenance pathways are enhanced in human embryonic stem cells, relative to differentiated human cells.

KW - Cell Differentiation

KW - Comet Assay

KW - DNA Damage

KW - DNA Repair

KW - Embryonic Stem Cells

KW - Fibroblasts

KW - Ficusin

KW - Hela Cells

KW - Humans

KW - Hydrogen Peroxide

KW - Oligonucleotide Array Sequence Analysis

KW - Radiation, Ionizing

KW - Ultraviolet Rays

U2 - 10.1634/stemcells.2007-1041

DO - 10.1634/stemcells.2007-1041

M3 - Journal article

C2 - 18566332

SN - 1066-5099

VL - 26

SP - 2266

EP - 2274

JO - Stem Cells

JF - Stem Cells

IS - 9

ER -