High-throughput sequencing of forensic genetic samples using punches of FTA cards with buccal swabs

TY - JOUR

T1 - High-throughput sequencing of forensic genetic samples using punches of FTA cards with buccal swabs

AU - Kampmann, Marie-Louise

AU - Buchard, Anders

AU - Børsting, Claus

AU - Morling, Niels

PY - 2016/9

Y1 - 2016/9

N2 - Here, we demonstrate that punches from buccal swab samples preserved on FTA cards can be used for high-throughput DNA sequencing, also known as massively parallel sequencing (MPS). We typed 44 reference samples with the HID-Ion AmpliSeq Identity Panel using washed 1.2 mm punches from FTA cards with buccal swabs and compared the results with those obtained with DNA extracted using the EZ1 DNA Investigator Kit. Concordant profiles were obtained for all samples. Our protocol includes simple punch, wash, and PCR steps, reducing cost and hands-on time in the laboratory. Furthermore, it facilitates automation of DNA sequencing.

AB - Here, we demonstrate that punches from buccal swab samples preserved on FTA cards can be used for high-throughput DNA sequencing, also known as massively parallel sequencing (MPS). We typed 44 reference samples with the HID-Ion AmpliSeq Identity Panel using washed 1.2 mm punches from FTA cards with buccal swabs and compared the results with those obtained with DNA extracted using the EZ1 DNA Investigator Kit. Concordant profiles were obtained for all samples. Our protocol includes simple punch, wash, and PCR steps, reducing cost and hands-on time in the laboratory. Furthermore, it facilitates automation of DNA sequencing.

U2 - 10.2144/000114453

DO - 10.2144/000114453

M3 - Journal article

C2 - 27625209

SN - 0736-6205

VL - 61

SP - 149

EP - 151

JO - BioTechniques

JF - BioTechniques

IS - 3

ER -