High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

Ana Kneževic; Jonathan Bones; Stjepan Kresimir Kracun; Olga Gornik; Pauline M Rudd; Gordan Lauc

doi:10.1039/c1an15684e

High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

Ana Kneževic, Jonathan Bones, Stjepan Kresimir Kracun, Olga Gornik, Pauline M Rudd, Gordan Lauc

Glycomics Program

28 Citationer (Scopus)

Abstract

A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.

Originalsprog	Engelsk
Tidsskrift	The Analyst
Vol/bind	136
Udgave nummer	22
Sider (fra-til)	4670-3
Antal sider	4
DOI	https://doi.org/10.1039/c1an15684e
Status	Udgivet - 21 nov. 2011

Adgang til dokumentet

10.1039/c1an15684e

Citationsformater

@article{1df8ed0334674f4ea8bdbae1af48e509,

title = "High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection",

abstract = "A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.",

keywords = "Chromatography, High Pressure Liquid, Glycomics, Humans, Polysaccharides, Spectrometry, Fluorescence, Staining and Labeling",

author = "Ana Kne{\v z}evic and Jonathan Bones and Kracun, {Stjepan Kresimir} and Olga Gornik and Rudd, {Pauline M} and Gordan Lauc",

year = "2011",

month = nov,

day = "21",

doi = "10.1039/c1an15684e",

language = "English",

volume = "136",

pages = "4670--3",

journal = "The Analyst",

number = "22",

}

TY - JOUR

T1 - High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

AU - Kneževic, Ana

AU - Bones, Jonathan

AU - Kracun, Stjepan Kresimir

AU - Gornik, Olga

AU - Rudd, Pauline M

AU - Lauc, Gordan

PY - 2011/11/21

Y1 - 2011/11/21

N2 - A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.

AB - A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.

KW - Chromatography, High Pressure Liquid

KW - Glycomics

KW - Humans

KW - Polysaccharides

KW - Spectrometry, Fluorescence

KW - Staining and Labeling

U2 - 10.1039/c1an15684e

DO - 10.1039/c1an15684e

M3 - Journal article

C2 - 21956149

VL - 136

SP - 4670

EP - 4673

JO - The Analyst

JF - The Analyst

IS - 22

ER -

High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater