High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

Ana Kneževic; Jonathan Bones; Stjepan Kresimir Kracun; Olga Gornik; Pauline M Rudd; Gordan Lauc

doi:10.1039/c1an15684e

High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

Ana Kneževic, Jonathan Bones, Stjepan Kresimir Kracun, Olga Gornik, Pauline M Rudd, Gordan Lauc

Glycomics Program

28 Citations (Scopus)

Abstract

A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.

Original language	English
Journal	The Analyst
Volume	136
Issue number	22
Pages (from-to)	4670-3
Number of pages	4
DOIs	https://doi.org/10.1039/c1an15684e
Publication status	Published - 21 Nov 2011

Keywords

Chromatography, High Pressure Liquid
Glycomics
Humans
Polysaccharides
Spectrometry, Fluorescence
Staining and Labeling

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10.1039/c1an15684e

Cite this

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abstract = "A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.",

keywords = "Chromatography, High Pressure Liquid, Glycomics, Humans, Polysaccharides, Spectrometry, Fluorescence, Staining and Labeling",

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AU - Kneževic, Ana

AU - Bones, Jonathan

AU - Kracun, Stjepan Kresimir

AU - Gornik, Olga

AU - Rudd, Pauline M

AU - Lauc, Gordan

PY - 2011/11/21

Y1 - 2011/11/21

N2 - A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.

AB - A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.

KW - Chromatography, High Pressure Liquid

KW - Glycomics

KW - Humans

KW - Polysaccharides

KW - Spectrometry, Fluorescence

KW - Staining and Labeling

U2 - 10.1039/c1an15684e

DO - 10.1039/c1an15684e

M3 - Journal article

C2 - 21956149

VL - 136

SP - 4670

EP - 4673

JO - The Analyst

JF - The Analyst

IS - 22

ER -

High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

Abstract

Keywords

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