Fictive locomotion in the adult decerebrate and spinal mouse in vivo

TY - JOUR

T1 - Fictive locomotion in the adult decerebrate and spinal mouse in vivo

AU - Meehan, Claire Francesca

AU - Grøndahl, Lillian

AU - Nielsen, Jens Bo

AU - Hultborn, Hans R

N1 - CURIS 2012 5200 007

PY - 2012/1

Y1 - 2012/1

N2 - Recently, transgenic mice have been created with mutations affecting the components of the mammalian spinal central pattern generator (CPG) for locomotion; however, it has currently only been possible to evoke fictive locomotion in mice, using neonatalin vitropreparations. Here, we demonstrate that it is possible to evoke fictive locomotion in the adult decerebrate mousein vivousing l-3,4-dihydroxyphenylalanine methyl ester hydrochloride (l-DOPA) and 5-hydroxytryptophan (5HTP) following injection of the monoaminoxiadase inhibitor Nialamide. We investigate the effects of afferent stimulation and spinalization as well as demonstrate the possibility of simultaneous intracellular recording of rhythmically active motoneurones. Our results demonstrate that several features of the mouse locomotor CPG are similar to those that have been observed in rat, cat, rabbit and monkey suggesting a fairly conserved organisation and allowing for future results in transgenic mice to be extrapolated to existing knowledge of CPG components and circuitry obtained in larger species.

AB - Recently, transgenic mice have been created with mutations affecting the components of the mammalian spinal central pattern generator (CPG) for locomotion; however, it has currently only been possible to evoke fictive locomotion in mice, using neonatalin vitropreparations. Here, we demonstrate that it is possible to evoke fictive locomotion in the adult decerebrate mousein vivousing l-3,4-dihydroxyphenylalanine methyl ester hydrochloride (l-DOPA) and 5-hydroxytryptophan (5HTP) following injection of the monoaminoxiadase inhibitor Nialamide. We investigate the effects of afferent stimulation and spinalization as well as demonstrate the possibility of simultaneous intracellular recording of rhythmically active motoneurones. Our results demonstrate that several features of the mouse locomotor CPG are similar to those that have been observed in rat, cat, rabbit and monkey suggesting a fairly conserved organisation and allowing for future results in transgenic mice to be extrapolated to existing knowledge of CPG components and circuitry obtained in larger species.

U2 - 10.1113/jphysiol.2011.214643

DO - 10.1113/jphysiol.2011.214643

M3 - Journal article

C2 - 22106172

SN - 0022-3751

VL - 590

SP - 289

EP - 300

JO - The Journal of Physiology

JF - The Journal of Physiology

IS - 2

ER -