TY - JOUR
T1 - FGF-2 low molecular weight selectively promotes neuritogenesis of motor neurons in vitro
AU - Allodi, Ilary
AU - Casals-Díaz, Laura
AU - Santos-Nogueira, Eva
AU - Gonzalez-Perez, Francisco
AU - Navarro, Xavier
AU - Udina, Esther
PY - 2013/4
Y1 - 2013/4
N2 - In this study, we screened in vitro the different capabilities of trophic factors with promising effect for enhancing selective regeneration and thus promoting specific reinnervation of target organs after peripheral nerve regeneration. We found that FGF-2 (18 kDa) was the trophic factor that exerted the most selective effect in promoting neurite outgrowth of spinal motoneurons both in terms of elongation and arborization. The mechanism underlying this effect on neuritogenesis seems related to FGF-2 enhancing the interaction between FGFR-1 and PSA-NCAM. The interaction of these two receptors is important during the early stages of neuritogenesis and pathfinding, while integrin alpha7B subunit seems to play a role during neurite stabilization.
AB - In this study, we screened in vitro the different capabilities of trophic factors with promising effect for enhancing selective regeneration and thus promoting specific reinnervation of target organs after peripheral nerve regeneration. We found that FGF-2 (18 kDa) was the trophic factor that exerted the most selective effect in promoting neurite outgrowth of spinal motoneurons both in terms of elongation and arborization. The mechanism underlying this effect on neuritogenesis seems related to FGF-2 enhancing the interaction between FGFR-1 and PSA-NCAM. The interaction of these two receptors is important during the early stages of neuritogenesis and pathfinding, while integrin alpha7B subunit seems to play a role during neurite stabilization.
KW - Animals
KW - Animals, Newborn
KW - Cells, Cultured
KW - Female
KW - Fibroblast Growth Factor 2/pharmacology
KW - Ganglia, Spinal/cytology
KW - Motor Neurons/cytology
KW - Neurites/drug effects
KW - Neurogenesis/drug effects
KW - Organ Culture Techniques
KW - Rats
KW - Rats, Sprague-Dawley
KW - Spinal Cord/cytology
U2 - 10.1007/s12035-012-8389-z
DO - 10.1007/s12035-012-8389-z
M3 - Journal article
C2 - 23275175
SN - 0893-7648
VL - 47
SP - 770
EP - 781
JO - Molecular Neurobiology
JF - Molecular Neurobiology
IS - 2
ER -