Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1

Nikolaj Kulahin; Christina Kasper; Michael Gajhede; Vladimir Berezin; Elisabeth Bock; Jette Sandholm Jensen Kastrup

doi:10.1107/S0907444904001167

Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1

Nikolaj Kulahin, Christina Kasper, Michael Gajhede, Vladimir Berezin, Elisabeth Bock, Jette Sandholm Jensen Kastrup

Eyepath Lab

7 Citationer (Scopus)

Abstract

Recombinant proteins consisting of either the four or five amino-terminal immunoglobulin (Ig) modules of the rat neural cell-adhesion molecule NCAM or the whole extracellular part [six Ig and five fibronectin type III (F3) modules] of mouse L1 have been expressed in Drosophila S2 cells. The proteins have been purified and crystallized. The crystals of the recombinant protein containing the four amino-terminal Ig modules of NCAM diffract X-rays to approximately 4 A resolution and belong to space group P622 or P6(3)22, with unit-cell parameters a = b = 258.7, c = 182.4 A. No diffraction was observed for the other two protein constructs. This is a step towards determining the structure of multimodular constructs of cell-adhesion molecules that exhibit high structural flexibility.

Originalsprog	Engelsk
Tidsskrift	Acta Crystallographica. Section D: Biological Crystallography
Vol/bind	60
Udgave nummer	Pt 3
Sider (fra-til)	591-3
Antal sider	3
ISSN	0907-4449
DOI	https://doi.org/10.1107/S0907444904001167
Status	Udgivet - mar. 2004

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10.1107/S0907444904001167

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Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1. / Kulahin, Nikolaj; Kasper, Christina; Gajhede, Michael et al.

I: Acta Crystallographica. Section D: Biological Crystallography, Bind 60, Nr. Pt 3, 03.2004, s. 591-3.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1",

abstract = "Recombinant proteins consisting of either the four or five amino-terminal immunoglobulin (Ig) modules of the rat neural cell-adhesion molecule NCAM or the whole extracellular part [six Ig and five fibronectin type III (F3) modules] of mouse L1 have been expressed in Drosophila S2 cells. The proteins have been purified and crystallized. The crystals of the recombinant protein containing the four amino-terminal Ig modules of NCAM diffract X-rays to approximately 4 A resolution and belong to space group P622 or P6(3)22, with unit-cell parameters a = b = 258.7, c = 182.4 A. No diffraction was observed for the other two protein constructs. This is a step towards determining the structure of multimodular constructs of cell-adhesion molecules that exhibit high structural flexibility.",

keywords = "Animals, Cell Line, Crystallization, Crystallography, X-Ray, Drosophila, Gene Expression, Mice, Neural Cell Adhesion Molecule L1, Protein Structure, Tertiary, Rats, Recombinant Proteins",

author = "Nikolaj Kulahin and Christina Kasper and Michael Gajhede and Vladimir Berezin and Elisabeth Bock and Kastrup, {Jette Sandholm Jensen}",

year = "2004",

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T1 - Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1

AU - Kulahin, Nikolaj

AU - Kasper, Christina

AU - Gajhede, Michael

AU - Berezin, Vladimir

AU - Bock, Elisabeth

AU - Kastrup, Jette Sandholm Jensen

PY - 2004/3

Y1 - 2004/3

N2 - Recombinant proteins consisting of either the four or five amino-terminal immunoglobulin (Ig) modules of the rat neural cell-adhesion molecule NCAM or the whole extracellular part [six Ig and five fibronectin type III (F3) modules] of mouse L1 have been expressed in Drosophila S2 cells. The proteins have been purified and crystallized. The crystals of the recombinant protein containing the four amino-terminal Ig modules of NCAM diffract X-rays to approximately 4 A resolution and belong to space group P622 or P6(3)22, with unit-cell parameters a = b = 258.7, c = 182.4 A. No diffraction was observed for the other two protein constructs. This is a step towards determining the structure of multimodular constructs of cell-adhesion molecules that exhibit high structural flexibility.

AB - Recombinant proteins consisting of either the four or five amino-terminal immunoglobulin (Ig) modules of the rat neural cell-adhesion molecule NCAM or the whole extracellular part [six Ig and five fibronectin type III (F3) modules] of mouse L1 have been expressed in Drosophila S2 cells. The proteins have been purified and crystallized. The crystals of the recombinant protein containing the four amino-terminal Ig modules of NCAM diffract X-rays to approximately 4 A resolution and belong to space group P622 or P6(3)22, with unit-cell parameters a = b = 258.7, c = 182.4 A. No diffraction was observed for the other two protein constructs. This is a step towards determining the structure of multimodular constructs of cell-adhesion molecules that exhibit high structural flexibility.

KW - Animals

KW - Cell Line

KW - Crystallization

KW - Crystallography, X-Ray

KW - Drosophila

KW - Gene Expression

KW - Mice

KW - Neural Cell Adhesion Molecule L1

KW - Protein Structure, Tertiary

KW - Rats

KW - Recombinant Proteins

U2 - 10.1107/S0907444904001167

DO - 10.1107/S0907444904001167

M3 - Journal article

C2 - 14993704

SN - 2059-7983

VL - 60

SP - 591

EP - 593

JO - Acta Crystallographica Section D: Structural Biology

JF - Acta Crystallographica Section D: Structural Biology

IS - Pt 3

ER -

Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1

Abstract

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