Exercise increases interleukin-10 levels both intraarticularly and peri-synovially in patients with knee osteoarthritis: a randomized controlled trial

Ida C Helmark; Ulla R Mikkelsen; Jens Børglum; Anders Rothe; Marie C H Petersen; Ove Andersen; Henning Langberg; Michael Kjær

doi:10.1186/ar3064

Exercise increases interleukin-10 levels both intraarticularly and peri-synovially in patients with knee osteoarthritis: a randomized controlled trial

Ida C Helmark, Ulla R Mikkelsen, Jens Børglum, Anders Rothe, Marie C H Petersen, Ove Andersen, Henning Langberg, Michael Kjær

112 Citationer (Scopus)

Abstract

Introduction: The microdialysis method was applied to the human knee joint with osteoarthritis (OA) in order to reveal changes in biochemical markers of cartilage and inflammation, intraarticularly and in the synovium, in response to a single bout of mechanical joint loading.Methods: Thirty-one female subjects with OA of the knee were randomized to non-exercise (NEx) or exercise (Ex) groups. Following acute resistance exercise (25 sets of 10 repetitions at 60% of 1 Repetition Maximum) or none (NEx), peripheral nerve blocks just below the inguinal ligament were applied and two microdialysis catheters were positioned in two different compartments, intraarticularly and peri-synovially. The microdialysis catheters were perfused at a slow rate (2 μl/minute) with a solution of Ringer-acetate and radioactively labelled glucose allowing for determination of relative recovery (RR) and calculation of interstitial concentrations of inflammatory and cartilage biomarkers over a three-hour period.Results: A significant increase of Interleukin (IL) -10 was discovered in both positions of the knee in the Ex group over the three hours post exercise, whereas IL-10 remained stationary over time in the NEx group. IL-6 and IL-8 displayed significant increases over time regardless of group and position of the catheter. Cartilage oligomeric matrix protein (COMP) decreased intraarticularly in the post exercise period in the Ex group compared to the NEx group.Conclusions: Exercise caused an increase in both intraarticular and peri-synovial concentrations of IL-10 in a group of human females with knee OA. This suggests a positive effect of exercise on a chondroprotective anti-inflammatory cytokine response in patients with knee OA and might contribute to explaining the beneficial effect that exercise has on OA. Trial registration: NCT01090375.

Originalsprog	Engelsk
Tidsskrift	Arthritis Research & Therapy
Vol/bind	12
Udgave nummer	4
Sider (fra-til)	R126
ISSN	1478-6362
DOI	https://doi.org/10.1186/ar3064
Status	Udgivet - 1 jul. 2010

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10.1186/ar3064

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title = "Exercise increases interleukin-10 levels both intraarticularly and peri-synovially in patients with knee osteoarthritis: a randomized controlled trial",

abstract = "Introduction: The microdialysis method was applied to the human knee joint with osteoarthritis (OA) in order to reveal changes in biochemical markers of cartilage and inflammation, intraarticularly and in the synovium, in response to a single bout of mechanical joint loading.Methods: Thirty-one female subjects with OA of the knee were randomized to non-exercise (NEx) or exercise (Ex) groups. Following acute resistance exercise (25 sets of 10 repetitions at 60% of 1 Repetition Maximum) or none (NEx), peripheral nerve blocks just below the inguinal ligament were applied and two microdialysis catheters were positioned in two different compartments, intraarticularly and peri-synovially. The microdialysis catheters were perfused at a slow rate (2 μl/minute) with a solution of Ringer-acetate and radioactively labelled glucose allowing for determination of relative recovery (RR) and calculation of interstitial concentrations of inflammatory and cartilage biomarkers over a three-hour period.Results: A significant increase of Interleukin (IL) -10 was discovered in both positions of the knee in the Ex group over the three hours post exercise, whereas IL-10 remained stationary over time in the NEx group. IL-6 and IL-8 displayed significant increases over time regardless of group and position of the catheter. Cartilage oligomeric matrix protein (COMP) decreased intraarticularly in the post exercise period in the Ex group compared to the NEx group.Conclusions: Exercise caused an increase in both intraarticular and peri-synovial concentrations of IL-10 in a group of human females with knee OA. This suggests a positive effect of exercise on a chondroprotective anti-inflammatory cytokine response in patients with knee OA and might contribute to explaining the beneficial effect that exercise has on OA. Trial registration: NCT01090375.",

keywords = "Aged, Arteries, Biological Markers, Cartilage, Articular, Exercise, Female, Humans, Interleukin-10, Microdialysis, Middle Aged, Models, Biological, Osteoarthritis, Knee, Resistance Training, Synovial Fluid, Weight-Bearing",

author = "Helmark, {Ida C} and Mikkelsen, {Ulla R} and Jens B{\o}rglum and Anders Rothe and Petersen, {Marie C H} and Ove Andersen and Henning Langberg and Michael Kj{\ae}r",

year = "2010",

month = jul,

day = "1",

doi = "10.1186/ar3064",

language = "English",

volume = "12",

pages = "R126",

journal = "Arthritis Research & Therapy",

issn = "1478-6354",

publisher = "BioMed Central",

number = "4",

}

TY - JOUR

T1 - Exercise increases interleukin-10 levels both intraarticularly and peri-synovially in patients with knee osteoarthritis

T2 - a randomized controlled trial

AU - Helmark, Ida C

AU - Mikkelsen, Ulla R

AU - Børglum, Jens

AU - Rothe, Anders

AU - Petersen, Marie C H

AU - Andersen, Ove

AU - Langberg, Henning

AU - Kjær, Michael

PY - 2010/7/1

Y1 - 2010/7/1

N2 - Introduction: The microdialysis method was applied to the human knee joint with osteoarthritis (OA) in order to reveal changes in biochemical markers of cartilage and inflammation, intraarticularly and in the synovium, in response to a single bout of mechanical joint loading.Methods: Thirty-one female subjects with OA of the knee were randomized to non-exercise (NEx) or exercise (Ex) groups. Following acute resistance exercise (25 sets of 10 repetitions at 60% of 1 Repetition Maximum) or none (NEx), peripheral nerve blocks just below the inguinal ligament were applied and two microdialysis catheters were positioned in two different compartments, intraarticularly and peri-synovially. The microdialysis catheters were perfused at a slow rate (2 μl/minute) with a solution of Ringer-acetate and radioactively labelled glucose allowing for determination of relative recovery (RR) and calculation of interstitial concentrations of inflammatory and cartilage biomarkers over a three-hour period.Results: A significant increase of Interleukin (IL) -10 was discovered in both positions of the knee in the Ex group over the three hours post exercise, whereas IL-10 remained stationary over time in the NEx group. IL-6 and IL-8 displayed significant increases over time regardless of group and position of the catheter. Cartilage oligomeric matrix protein (COMP) decreased intraarticularly in the post exercise period in the Ex group compared to the NEx group.Conclusions: Exercise caused an increase in both intraarticular and peri-synovial concentrations of IL-10 in a group of human females with knee OA. This suggests a positive effect of exercise on a chondroprotective anti-inflammatory cytokine response in patients with knee OA and might contribute to explaining the beneficial effect that exercise has on OA. Trial registration: NCT01090375.

AB - Introduction: The microdialysis method was applied to the human knee joint with osteoarthritis (OA) in order to reveal changes in biochemical markers of cartilage and inflammation, intraarticularly and in the synovium, in response to a single bout of mechanical joint loading.Methods: Thirty-one female subjects with OA of the knee were randomized to non-exercise (NEx) or exercise (Ex) groups. Following acute resistance exercise (25 sets of 10 repetitions at 60% of 1 Repetition Maximum) or none (NEx), peripheral nerve blocks just below the inguinal ligament were applied and two microdialysis catheters were positioned in two different compartments, intraarticularly and peri-synovially. The microdialysis catheters were perfused at a slow rate (2 μl/minute) with a solution of Ringer-acetate and radioactively labelled glucose allowing for determination of relative recovery (RR) and calculation of interstitial concentrations of inflammatory and cartilage biomarkers over a three-hour period.Results: A significant increase of Interleukin (IL) -10 was discovered in both positions of the knee in the Ex group over the three hours post exercise, whereas IL-10 remained stationary over time in the NEx group. IL-6 and IL-8 displayed significant increases over time regardless of group and position of the catheter. Cartilage oligomeric matrix protein (COMP) decreased intraarticularly in the post exercise period in the Ex group compared to the NEx group.Conclusions: Exercise caused an increase in both intraarticular and peri-synovial concentrations of IL-10 in a group of human females with knee OA. This suggests a positive effect of exercise on a chondroprotective anti-inflammatory cytokine response in patients with knee OA and might contribute to explaining the beneficial effect that exercise has on OA. Trial registration: NCT01090375.

KW - Aged

KW - Arteries

KW - Biological Markers

KW - Cartilage, Articular

KW - Exercise

KW - Female

KW - Humans

KW - Interleukin-10

KW - Microdialysis

KW - Middle Aged

KW - Models, Biological

KW - Osteoarthritis, Knee

KW - Resistance Training

KW - Synovial Fluid

KW - Weight-Bearing

U2 - 10.1186/ar3064

DO - 10.1186/ar3064

M3 - Journal article

C2 - 20594330

SN - 1478-6354

VL - 12

SP - R126

JO - Arthritis Research & Therapy

JF - Arthritis Research & Therapy

IS - 4

ER -

Exercise increases interleukin-10 levels both intraarticularly and peri-synovially in patients with knee osteoarthritis: a randomized controlled trial

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