[Pasteurella] caballi infection not limited to horses - a closer look at taxon 42 of Bisgaard

Henrik Christensen; J. Hommez; John Elmerdahl Olsen; Magne Bisgaard

doi:10.1111/j.1472-765X.2006.01971.x

[Pasteurella] caballi infection not limited to horses - a closer look at taxon 42 of Bisgaard

Henrik Christensen, J. Hommez, John Elmerdahl Olsen, Magne Bisgaard

5 Citationer (Scopus)

Abstract

Aim To investigate if taxon 42 of Bisgaard isolated from pigs represents genuine [Pasteurella] caballi which has previously only been isolated from horses. Methods and Results A total of 15 field isolates from horses and pigs from 5 different countries representing three continents were to subjected extended phenotypical characterization. Although minor differences were observed between taxon 42 and [P.] caballi, these differences did not allow phenotypic separation. Ribotyping based on HindIII digestion showed five profiles based on 9 band positions. One [P.] caballi strain and two taxon 42 strains shared the same profile. Ribotyping using HpaII gave a higher diversity with 9 profiles based on 10 band positions. While no profiles were shared between the Taxon 42 and [P.] caballi strains, pattern analysis showed that two of the Taxon 42 isolates were most similar (91 % similarity) with a [P.] caballi isolate. 16S rRNA gene sequencing of one strain of taxon 42 and one strain of [P.] caballi was performed and compared to the published sequence for the type strain of [P.] caballi. The three strains showed nearly identical sequences with at least 99.8 % similarity. DNA re-associations measured by the micro-well method were 79 and 77 %, respectively between the type strain of [P.] caballi and two strains of taxon 42 representing distinct ribotypes and confirmed that taxon 42 belongs to [P.] caballi. Conclusion The present investigation documents that [P.] caballi can be isolated from clinical respiratory specimens from pigs as well as the recognised association with respiratory infections in horses and horse bite infection in humans. Strains classified as taxon 42 are [P.] caballi isolated from pigs and for both pigs and horses lesions mainly include the respiratory tract. Significance and impact of study The results will improve the diagnostics and progress studies of virulence and epidemiology of [P.] caballi.

Originalsprog	Engelsk
Tidsskrift	Letters in Applied Microbiology
Vol/bind	43
Udgave nummer	4
Sider (fra-til)	424-429
Antal sider	6
ISSN	0266-8254
DOI	https://doi.org/10.1111/j.1472-765X.2006.01971.x
Status	Udgivet - 2006

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10.1111/j.1472-765X.2006.01971.x

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title = "[Pasteurella] caballi infection not limited to horses - a closer look at taxon 42 of Bisgaard",

abstract = "Aim To investigate if taxon 42 of Bisgaard isolated from pigs represents genuine [Pasteurella] caballi which has previously only been isolated from horses. Methods and Results A total of 15 field isolates from horses and pigs from 5 different countries representing three continents were to subjected extended phenotypical characterization. Although minor differences were observed between taxon 42 and [P.] caballi, these differences did not allow phenotypic separation. Ribotyping based on HindIII digestion showed five profiles based on 9 band positions. One [P.] caballi strain and two taxon 42 strains shared the same profile. Ribotyping using HpaII gave a higher diversity with 9 profiles based on 10 band positions. While no profiles were shared between the Taxon 42 and [P.] caballi strains, pattern analysis showed that two of the Taxon 42 isolates were most similar (91 % similarity) with a [P.] caballi isolate. 16S rRNA gene sequencing of one strain of taxon 42 and one strain of [P.] caballi was performed and compared to the published sequence for the type strain of [P.] caballi. The three strains showed nearly identical sequences with at least 99.8 % similarity. DNA re-associations measured by the micro-well method were 79 and 77 %, respectively between the type strain of [P.] caballi and two strains of taxon 42 representing distinct ribotypes and confirmed that taxon 42 belongs to [P.] caballi. Conclusion The present investigation documents that [P.] caballi can be isolated from clinical respiratory specimens from pigs as well as the recognised association with respiratory infections in horses and horse bite infection in humans. Strains classified as taxon 42 are [P.] caballi isolated from pigs and for both pigs and horses lesions mainly include the respiratory tract. Significance and impact of study The results will improve the diagnostics and progress studies of virulence and epidemiology of [P.] caballi.",

author = "Henrik Christensen and J. Hommez and Olsen, {John Elmerdahl} and Magne Bisgaard",

year = "2006",

doi = "10.1111/j.1472-765X.2006.01971.x",

language = "English",

volume = "43",

pages = "424--429",

journal = "Proceedings of the Society for Applied Bacteriology",

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T1 - [Pasteurella] caballi infection not limited to horses - a closer look at taxon 42 of Bisgaard

AU - Christensen, Henrik

AU - Hommez, J.

AU - Olsen, John Elmerdahl

AU - Bisgaard, Magne

PY - 2006

Y1 - 2006

N2 - Aim To investigate if taxon 42 of Bisgaard isolated from pigs represents genuine [Pasteurella] caballi which has previously only been isolated from horses. Methods and Results A total of 15 field isolates from horses and pigs from 5 different countries representing three continents were to subjected extended phenotypical characterization. Although minor differences were observed between taxon 42 and [P.] caballi, these differences did not allow phenotypic separation. Ribotyping based on HindIII digestion showed five profiles based on 9 band positions. One [P.] caballi strain and two taxon 42 strains shared the same profile. Ribotyping using HpaII gave a higher diversity with 9 profiles based on 10 band positions. While no profiles were shared between the Taxon 42 and [P.] caballi strains, pattern analysis showed that two of the Taxon 42 isolates were most similar (91 % similarity) with a [P.] caballi isolate. 16S rRNA gene sequencing of one strain of taxon 42 and one strain of [P.] caballi was performed and compared to the published sequence for the type strain of [P.] caballi. The three strains showed nearly identical sequences with at least 99.8 % similarity. DNA re-associations measured by the micro-well method were 79 and 77 %, respectively between the type strain of [P.] caballi and two strains of taxon 42 representing distinct ribotypes and confirmed that taxon 42 belongs to [P.] caballi. Conclusion The present investigation documents that [P.] caballi can be isolated from clinical respiratory specimens from pigs as well as the recognised association with respiratory infections in horses and horse bite infection in humans. Strains classified as taxon 42 are [P.] caballi isolated from pigs and for both pigs and horses lesions mainly include the respiratory tract. Significance and impact of study The results will improve the diagnostics and progress studies of virulence and epidemiology of [P.] caballi.

AB - Aim To investigate if taxon 42 of Bisgaard isolated from pigs represents genuine [Pasteurella] caballi which has previously only been isolated from horses. Methods and Results A total of 15 field isolates from horses and pigs from 5 different countries representing three continents were to subjected extended phenotypical characterization. Although minor differences were observed between taxon 42 and [P.] caballi, these differences did not allow phenotypic separation. Ribotyping based on HindIII digestion showed five profiles based on 9 band positions. One [P.] caballi strain and two taxon 42 strains shared the same profile. Ribotyping using HpaII gave a higher diversity with 9 profiles based on 10 band positions. While no profiles were shared between the Taxon 42 and [P.] caballi strains, pattern analysis showed that two of the Taxon 42 isolates were most similar (91 % similarity) with a [P.] caballi isolate. 16S rRNA gene sequencing of one strain of taxon 42 and one strain of [P.] caballi was performed and compared to the published sequence for the type strain of [P.] caballi. The three strains showed nearly identical sequences with at least 99.8 % similarity. DNA re-associations measured by the micro-well method were 79 and 77 %, respectively between the type strain of [P.] caballi and two strains of taxon 42 representing distinct ribotypes and confirmed that taxon 42 belongs to [P.] caballi. Conclusion The present investigation documents that [P.] caballi can be isolated from clinical respiratory specimens from pigs as well as the recognised association with respiratory infections in horses and horse bite infection in humans. Strains classified as taxon 42 are [P.] caballi isolated from pigs and for both pigs and horses lesions mainly include the respiratory tract. Significance and impact of study The results will improve the diagnostics and progress studies of virulence and epidemiology of [P.] caballi.

U2 - 10.1111/j.1472-765X.2006.01971.x

DO - 10.1111/j.1472-765X.2006.01971.x

M3 - Journal article

C2 - 16965374

SN - 0266-8254

VL - 43

SP - 424

EP - 429

JO - Proceedings of the Society for Applied Bacteriology

JF - Proceedings of the Society for Applied Bacteriology

IS - 4

ER -

[Pasteurella] caballi infection not limited to horses - a closer look at taxon 42 of Bisgaard

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