Electromembrane extraction with solvent modification of the acceptor solution: improved mass transfer of drugs of abuse from human plasma

Linda Vårdal; Elisabeth Leere Øiestad; Astrid Gjelstad; Henrik Jensen; Stig Pedersen-Bjergaard

Electromembrane extraction with solvent modification of the acceptor solution: improved mass transfer of drugs of abuse from human plasma

Linda Vårdal, Elisabeth Leere Øiestad, Astrid Gjelstad, Henrik Jensen, Stig Pedersen-Bjergaard

5 Citationer (Scopus)

Abstract

Aim: Electromembrane extraction (EME) of 37 drugs of abuse with significant differences in terms of polarity (0.68 < log P < 4.3) and basicity (1.17 < pK_a < 10.38) was investigated from human plasma. Materials & methods: EME was performed with 250 mM trifluoroacetic acid and DMSO (1:1 v/v) in the acceptor solution. Results & conclusion: The analytes were extracted efficiently with pure 2-nitrophenyloctyl ether as supported liquid membrane when the acceptor solution was modified with DMSO. Thus, using DMSO mixed with 250 mM trifluoroacetic acid (1:1, v/v) as acceptor solution, recoveries from 40 to 105% (relative standard deviation <20%) were obtained for 33 of the analytes under optimized conditions. EME followed by ultra-HPLC-MS/MS analysis was evaluated from human plasma, and the results were satisfactory.

Originalsprog	Engelsk
Tidsskrift	Bioanalysis
Vol/bind	11
Sider (fra-til)	755-771
ISSN	1757-6180
Status	Udgivet - apr. 2019

Citationsformater

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title = "Electromembrane extraction with solvent modification of the acceptor solution: improved mass transfer of drugs of abuse from human plasma",

abstract = "Aim: Electromembrane extraction (EME) of 37 drugs of abuse with significant differences in terms of polarity (0.68 < log P < 4.3) and basicity (1.17 < pKa < 10.38) was investigated from human plasma. Materials & methods: EME was performed with 250 mM trifluoroacetic acid and DMSO (1:1 v/v) in the acceptor solution. Results & conclusion: The analytes were extracted efficiently with pure 2-nitrophenyloctyl ether as supported liquid membrane when the acceptor solution was modified with DMSO. Thus, using DMSO mixed with 250 mM trifluoroacetic acid (1:1, v/v) as acceptor solution, recoveries from 40 to 105% (relative standard deviation <20%) were obtained for 33 of the analytes under optimized conditions. EME followed by ultra-HPLC-MS/MS analysis was evaluated from human plasma, and the results were satisfactory.",

author = "Linda V{\aa}rdal and {\O}iestad, {Elisabeth Leere} and Astrid Gjelstad and Henrik Jensen and Stig Pedersen-Bjergaard",

year = "2019",

month = apr,

language = "English",

volume = "11",

pages = "755--771",

journal = "Bioanalysis",

issn = "1757-6180",

publisher = "Future Science",

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TY - JOUR

T1 - Electromembrane extraction with solvent modification of the acceptor solution: improved mass transfer of drugs of abuse from human plasma

AU - Vårdal, Linda

AU - Øiestad, Elisabeth Leere

AU - Gjelstad, Astrid

AU - Jensen, Henrik

AU - Pedersen-Bjergaard, Stig

PY - 2019/4

Y1 - 2019/4

N2 - Aim: Electromembrane extraction (EME) of 37 drugs of abuse with significant differences in terms of polarity (0.68 < log P < 4.3) and basicity (1.17 < pKa < 10.38) was investigated from human plasma. Materials & methods: EME was performed with 250 mM trifluoroacetic acid and DMSO (1:1 v/v) in the acceptor solution. Results & conclusion: The analytes were extracted efficiently with pure 2-nitrophenyloctyl ether as supported liquid membrane when the acceptor solution was modified with DMSO. Thus, using DMSO mixed with 250 mM trifluoroacetic acid (1:1, v/v) as acceptor solution, recoveries from 40 to 105% (relative standard deviation <20%) were obtained for 33 of the analytes under optimized conditions. EME followed by ultra-HPLC-MS/MS analysis was evaluated from human plasma, and the results were satisfactory.

AB - Aim: Electromembrane extraction (EME) of 37 drugs of abuse with significant differences in terms of polarity (0.68 < log P < 4.3) and basicity (1.17 < pKa < 10.38) was investigated from human plasma. Materials & methods: EME was performed with 250 mM trifluoroacetic acid and DMSO (1:1 v/v) in the acceptor solution. Results & conclusion: The analytes were extracted efficiently with pure 2-nitrophenyloctyl ether as supported liquid membrane when the acceptor solution was modified with DMSO. Thus, using DMSO mixed with 250 mM trifluoroacetic acid (1:1, v/v) as acceptor solution, recoveries from 40 to 105% (relative standard deviation <20%) were obtained for 33 of the analytes under optimized conditions. EME followed by ultra-HPLC-MS/MS analysis was evaluated from human plasma, and the results were satisfactory.

M3 - Journal article

SN - 1757-6180

VL - 11

SP - 755

EP - 771

JO - Bioanalysis

JF - Bioanalysis

ER -

Electromembrane extraction with solvent modification of the acceptor solution: improved mass transfer of drugs of abuse from human plasma

Abstract

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