Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood

TY - JOUR

T1 - Effect of storage temperature in a Cambodian field setting on the fatty acid composition in whole blood

AU - Nurhasan, Mulia

AU - Roos, Nanna

AU - Aristizabal Henao, J J

AU - Chamnan, C

AU - Stark, K D

AU - Lauritzen, Lotte

N1 - CURIS 2015 NEXS 098

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Fatty acid analysis requires standardized collection and storage of samples, which can be a challenge under field conditions. This study describes the effect of storage temperature on fatty acid composition in two sets of whole blood samples collected from 66 children in a rural area in Cambodia. The samples were stored with butylated hydroxytoluene at -20°C and -80°C and the latter required extra transfers due to storage facility limitation. Fatty acid composition was analyzed by high-throughput gas-chromatography and evaluated by paired t-tests and Bland-Altman plots. Total amounts of fat in -20°C and -80°C samples did not differ, but there was relatively more highly unsaturated fatty acids (15.8±2.7 vs. 14.4±2.5%, p<0.001) and a lower n-6/n-3 ratio (6.4±1.4 vs. 6.9±1.4, p<0.001) in the -20°C samples. Our results indicate that the importance of storage temperature should be evaluated in the context of storage facility availability and risk of temperature fluctuations during transport.

AB - Fatty acid analysis requires standardized collection and storage of samples, which can be a challenge under field conditions. This study describes the effect of storage temperature on fatty acid composition in two sets of whole blood samples collected from 66 children in a rural area in Cambodia. The samples were stored with butylated hydroxytoluene at -20°C and -80°C and the latter required extra transfers due to storage facility limitation. Fatty acid composition was analyzed by high-throughput gas-chromatography and evaluated by paired t-tests and Bland-Altman plots. Total amounts of fat in -20°C and -80°C samples did not differ, but there was relatively more highly unsaturated fatty acids (15.8±2.7 vs. 14.4±2.5%, p<0.001) and a lower n-6/n-3 ratio (6.4±1.4 vs. 6.9±1.4, p<0.001) in the -20°C samples. Our results indicate that the importance of storage temperature should be evaluated in the context of storage facility availability and risk of temperature fluctuations during transport.

U2 - 10.1016/j.plefa.2015.02.001

DO - 10.1016/j.plefa.2015.02.001

M3 - Journal article

C2 - 25753812

SN - 0952-3278

VL - 96

SP - 57

EP - 61

JO - Prostaglandins, Leukotrienes & Essential Fatty Acids

JF - Prostaglandins, Leukotrienes & Essential Fatty Acids

IS - 1

ER -