Effect of next-generation sequencing in preimplantation genetic testing on live birth ratio

TY - JOUR

T1 - Effect of next-generation sequencing in preimplantation genetic testing on live birth ratio

AU - Liss, Joanna

AU - Pastuszek, Ewa

AU - Pukszta, Sebastian

AU - Hoffmann, Eva

AU - Kuczynski, Waldemar

AU - Lukaszuk, Aron

AU - Lukaszuk, Krzysztof

PY - 2018

Y1 - 2018

N2 - The present study analysed live birth ratios in frozen embryo transfer (FET) cycles where embryo ploidy status was determined with preimplantation genetic testing (PGT) using next-generation sequencing (NGS). PGT was performed on trophectoderm cells biopsied at the blastocyst stage. The present prospective cohort study included 112 women undergoing frozen embryo transfer, with NGS PGT. The control group consisted of 85 patients who underwent the IVF procedure with FET planned for a subsequent cycle. The live birth rate per cycle was higher by ∼18.5 percentage points in the investigated compared with control group (42.0% vs 23.5% respectively; P = 0.012). The differences between the study and control groups were also significant for clinical pregnancy (42.0% vs 23.5% respectively; P = 0.012), implantation (41.2% vs 22.2% respectively; P = 0.001) and pregnancy loss rates (9.6% vs 28.6% respectively; P = 0.027). The results show that PGT NGS is a useful method for embryo selection and it may be implemented in routine clinical practice with propitious results.

AB - The present study analysed live birth ratios in frozen embryo transfer (FET) cycles where embryo ploidy status was determined with preimplantation genetic testing (PGT) using next-generation sequencing (NGS). PGT was performed on trophectoderm cells biopsied at the blastocyst stage. The present prospective cohort study included 112 women undergoing frozen embryo transfer, with NGS PGT. The control group consisted of 85 patients who underwent the IVF procedure with FET planned for a subsequent cycle. The live birth rate per cycle was higher by ∼18.5 percentage points in the investigated compared with control group (42.0% vs 23.5% respectively; P = 0.012). The differences between the study and control groups were also significant for clinical pregnancy (42.0% vs 23.5% respectively; P = 0.012), implantation (41.2% vs 22.2% respectively; P = 0.001) and pregnancy loss rates (9.6% vs 28.6% respectively; P = 0.027). The results show that PGT NGS is a useful method for embryo selection and it may be implemented in routine clinical practice with propitious results.

KW - Additional keywords: frozen embryo transfer.

U2 - 10.1071/RD17428

DO - 10.1071/RD17428

M3 - Journal article

C2 - 29929575

AN - SCOPUS:85049229680

SN - 1031-3613

VL - 30

SP - 1720

EP - 1727

JO - Reproduction, Fertility and Development

JF - Reproduction, Fertility and Development

IS - 12

ER -