Effect of Lactobacillus salivarius Ls-33 on fecal microbiota in obese adolescents

TY - JOUR

T1 - Effect of Lactobacillus salivarius Ls-33 on fecal microbiota in obese adolescents

AU - Larsen, Nadja

AU - Vogensen, Finn Kvist

AU - Gøbel, Rikke Juul

AU - Michaelsen, Kim F.

AU - Forssten, Sofia D.

AU - Lahtinen, Sampo J.

AU - Jakobsen, Mogens

N1 - CURIS 2013 NEXS 136

PY - 2013/12

Y1 - 2013/12

N2 - Background & aims: This study is a part of the clinical trials with probiotic bacterium Lactobacillus salivarius Ls-33 conducted in obese adolescents. Previously reported clinical studies showed no effect of Ls-33 consumption on the metabolic syndrome in the subject group. The aim of the study was to investigate the impact of L. salivarius Ls-33 on fecal microbiota in obese adolescents. Methods: The study was a double-blinded intervention with 50 subjects randomized to intake of L. salivarius Ls-33 or placebo for 12 weeks. The fecal microbiota was assessed by real-time quantitative PCR before and after intervention. Concentrations of fecal short chain fatty acids were determined using gas chromatography. Results: Ratios of Bacteroides-Prevotella-Porphyromonas group to Firmicutes belonging bacteria, including Clostridium cluster XIV, Blautia coccoides_. Eubacteria rectale group and Roseburia intestinalis, were significantly increased (p≤0.05) after administration of Ls-33. The cell numbers of fecal bacteria, including the groups above as well as Clostridium cluster I, Clostridium cluster IV, Faecalibacterium prausnitzii, Enterobacteriaceae, Enterococcus, the Lactobacillus group and Bifidobacterium were not significantly altered by intervention. Similarly, short chain fatty acids remained unaffected. Conclusion: L. salivarius Ls-33 might modify the fecal microbiota in obese adolescents in a way not related to metabolic syndrome.Clinical trial number: NCT 01020617.

AB - Background & aims: This study is a part of the clinical trials with probiotic bacterium Lactobacillus salivarius Ls-33 conducted in obese adolescents. Previously reported clinical studies showed no effect of Ls-33 consumption on the metabolic syndrome in the subject group. The aim of the study was to investigate the impact of L. salivarius Ls-33 on fecal microbiota in obese adolescents. Methods: The study was a double-blinded intervention with 50 subjects randomized to intake of L. salivarius Ls-33 or placebo for 12 weeks. The fecal microbiota was assessed by real-time quantitative PCR before and after intervention. Concentrations of fecal short chain fatty acids were determined using gas chromatography. Results: Ratios of Bacteroides-Prevotella-Porphyromonas group to Firmicutes belonging bacteria, including Clostridium cluster XIV, Blautia coccoides_. Eubacteria rectale group and Roseburia intestinalis, were significantly increased (p≤0.05) after administration of Ls-33. The cell numbers of fecal bacteria, including the groups above as well as Clostridium cluster I, Clostridium cluster IV, Faecalibacterium prausnitzii, Enterobacteriaceae, Enterococcus, the Lactobacillus group and Bifidobacterium were not significantly altered by intervention. Similarly, short chain fatty acids remained unaffected. Conclusion: L. salivarius Ls-33 might modify the fecal microbiota in obese adolescents in a way not related to metabolic syndrome.Clinical trial number: NCT 01020617.

U2 - 10.1016/j.clnu.2013.02.007

DO - 10.1016/j.clnu.2013.02.007

M3 - Journal article

SN - 0261-5614

VL - 32

SP - 935

EP - 940

JO - Clinical Nutrition

JF - Clinical Nutrition

IS - 6

ER -