Abstract
Lysis of marine bacteria by viruses releases a range of organic compounds into the environment, including D- and L-amino acids, but the uptake of these compounds by other bacteria is not well characterized. This study determined that Photobacterium sp. strain SKA34 (Gamma - proteobacteria) increased in abundance following uptake of D- and L-amino acids from viral lysate of Cellulophaga sp. strain MM#3 (Flavobacteria). Ammonium and dissolved free amino acids were taken up almost to detection limits, suggesting that the C:N ratio of bioavailable organic matter in the lysate was high for Photobacterium sp. growth, thus causing a net uptake of ammonium. In contrast, only 1.51 μmol l−1 of the 4.77 μmol l−1 of the total dissolved combined amino acids (DCAAs) were taken up, indicating that a fraction of lysate-derived DCAAs were semi-labile or refractory to bacterial uptake. Both D- and L-amino acid uptake rates were approximately proportional to their concentrations, indicating similar availability for each enantiomer and unsaturated uptake rates. These results imply that under high C:N conditions, both D-amino acids (mainly found in bacterial cell walls) and L-amino acids (found in proteins of the rest of the cell) are equally available for bacterial growth, and support arguments that viruses are key players in marine nitrogen cycling.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Aquatic Microbial Ecology |
| Vol/bind | 73 |
| Udgave nummer | 3 |
| Sider (fra-til) | 235-243 |
| Antal sider | 9 |
| ISSN | 0948-3055 |
| DOI | |
| Status | Udgivet - 2014 |
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