TY - GEN
T1 - Development of a simple in-vial liquid-phase microextraction device for drug analysis compatible with capillary gas chromatography, capillary electrophoresis and high-performance liquid chromatography
AU - Rasmussen, Knut Einar
AU - Pedersen-Bjergaard, Stig
AU - Krogh, Mette
AU - Grefslie Ugland, Hege
AU - Grønhaug, Trine
PY - 2000/3/17
Y1 - 2000/3/17
N2 - A simple, inexpensive and disposable device for liquid-phase microextraction (LPME) is presented for use in combination with capillary gas chromatography (GC), capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC). 1-4 ml samples of human urine or plasma were filled into conventional 4-ml vials, whereafter 15-25 μl of the extraction medium (acceptor solution) was filled into a short piece of a porous hollow fiber and placed into the sample vial. The drugs of interest were extracted from the sample solutions and into the small volumes of acceptor solution based on high partition coefficients and were preconcentrated by a factor of 30-125. For LPME in combination with GC, the porous hollow fiber was filled with 15 μl n-octanol as the acceptor solution. Following 30 min of extraction, the organic acceptor solution was injected directly into the GC system. For LPME in combination with CE and HPLC, n-octanol was immobilized within the pores of the hollow fiber, while the internal volume of the fiber was filled with either 25 μl of 0.1 M HCl (for extraction of basic compounds) or 25 μl 0.02 M NaOH (for acidic compounds). Following 45 min extraction, the aqueous acceptor solution was injected directly into the CE or HPLC system. Owing to the low cost, the extraction devices were disposed after a single extraction which eliminated the possibility of carry over effects. In addition, because no expensive instrumentation was required for LPME, 10-30 samples were extracted in parallel to provide a high number of samples per unit time capacity. (C) 2000 Elsevier Science B.V.
AB - A simple, inexpensive and disposable device for liquid-phase microextraction (LPME) is presented for use in combination with capillary gas chromatography (GC), capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC). 1-4 ml samples of human urine or plasma were filled into conventional 4-ml vials, whereafter 15-25 μl of the extraction medium (acceptor solution) was filled into a short piece of a porous hollow fiber and placed into the sample vial. The drugs of interest were extracted from the sample solutions and into the small volumes of acceptor solution based on high partition coefficients and were preconcentrated by a factor of 30-125. For LPME in combination with GC, the porous hollow fiber was filled with 15 μl n-octanol as the acceptor solution. Following 30 min of extraction, the organic acceptor solution was injected directly into the GC system. For LPME in combination with CE and HPLC, n-octanol was immobilized within the pores of the hollow fiber, while the internal volume of the fiber was filled with either 25 μl of 0.1 M HCl (for extraction of basic compounds) or 25 μl 0.02 M NaOH (for acidic compounds). Following 45 min extraction, the aqueous acceptor solution was injected directly into the CE or HPLC system. Owing to the low cost, the extraction devices were disposed after a single extraction which eliminated the possibility of carry over effects. In addition, because no expensive instrumentation was required for LPME, 10-30 samples were extracted in parallel to provide a high number of samples per unit time capacity. (C) 2000 Elsevier Science B.V.
KW - Amphetamines
KW - Benzodiazepines
KW - Extraction methods
KW - Hollow fibers
KW - Liquid-phase microextraction
KW - Nonsteroidal anti-inflammatory drugs
UR - http://www.scopus.com/inward/record.url?scp=0034002699&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(99)01163-2
DO - 10.1016/S0021-9673(99)01163-2
M3 - Conference article
C2 - 10757280
AN - SCOPUS:0034002699
SN - 0301-4770
VL - 873
SP - 3
EP - 11
JO - Journal of Chromatography
JF - Journal of Chromatography
IS - 1
T2 - ExTech Symposium: Advances in Extraction Technologies
Y2 - 26 April 1999 through 30 April 1999
ER -