Comparative assessment of peptide concentration in milk protein hydrolysates and fractions

Jeanette Otte; M. Abdel-Hamid; A. Osman

doi:10.3923/ijds.2015.228.235

Comparative assessment of peptide concentration in milk protein hydrolysates and fractions

Jeanette Otte, M. Abdel-Hamid, A. Osman

Fødevarekemi

5 Citationer (Scopus)

Abstract

A comparative study using the Kjeldahl method and two colorimetric methods for the determination of total protein/peptide concentrations in a range of intact and hydrolysed milk protein was carried out. The samples were goat whey, camel whey, buffalos milk retentate and hydrolysates of these made with different enzymes as well as hydrolysate fractions. Bicinchoninic acid (BCA) and o-phthaldialdehyde (OPA) were used as coloring agents to specifically react with proteins and peptides. The results obtained with the BCA method and bovine serum albumin as standard did not correlate with the results obtained by the Kjeldahl method (r² = 0.17). The OPA method showed better agreement with the Kjeldahl method (r² = 0.69) and with the use of a whey protein hydrolysate as standard good estimates of the peptide contents in the hydrolysed milk protein samples was obtained compared to the results obtained with the Kjeldahl method.

Originalsprog	Engelsk
Tidsskrift	International Journal of Dairy Science
Vol/bind	10
Udgave nummer	5
Sider (fra-til)	228-235
Antal sider	8
ISSN	1811-9743
DOI	https://doi.org/10.3923/ijds.2015.228.235
Status	Udgivet - 21 sep. 2015

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10.3923/ijds.2015.228.235

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Citationsformater

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abstract = "A comparative study using the Kjeldahl method and two colorimetric methods for the determination of total protein/peptide concentrations in a range of intact and hydrolysed milk protein was carried out. The samples were goat whey, camel whey, buffalos milk retentate and hydrolysates of these made with different enzymes as well as hydrolysate fractions. Bicinchoninic acid (BCA) and o-phthaldialdehyde (OPA) were used as coloring agents to specifically react with proteins and peptides. The results obtained with the BCA method and bovine serum albumin as standard did not correlate with the results obtained by the Kjeldahl method (r2 = 0.17). The OPA method showed better agreement with the Kjeldahl method (r2 = 0.69) and with the use of a whey protein hydrolysate as standard good estimates of the peptide contents in the hydrolysed milk protein samples was obtained compared to the results obtained with the Kjeldahl method.",

author = "Jeanette Otte and M. Abdel-Hamid and A. Osman",

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T1 - Comparative assessment of peptide concentration in milk protein hydrolysates and fractions

AU - Otte, Jeanette

AU - Abdel-Hamid, M.

AU - Osman, A.

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Y1 - 2015/9/21

N2 - A comparative study using the Kjeldahl method and two colorimetric methods for the determination of total protein/peptide concentrations in a range of intact and hydrolysed milk protein was carried out. The samples were goat whey, camel whey, buffalos milk retentate and hydrolysates of these made with different enzymes as well as hydrolysate fractions. Bicinchoninic acid (BCA) and o-phthaldialdehyde (OPA) were used as coloring agents to specifically react with proteins and peptides. The results obtained with the BCA method and bovine serum albumin as standard did not correlate with the results obtained by the Kjeldahl method (r2 = 0.17). The OPA method showed better agreement with the Kjeldahl method (r2 = 0.69) and with the use of a whey protein hydrolysate as standard good estimates of the peptide contents in the hydrolysed milk protein samples was obtained compared to the results obtained with the Kjeldahl method.

AB - A comparative study using the Kjeldahl method and two colorimetric methods for the determination of total protein/peptide concentrations in a range of intact and hydrolysed milk protein was carried out. The samples were goat whey, camel whey, buffalos milk retentate and hydrolysates of these made with different enzymes as well as hydrolysate fractions. Bicinchoninic acid (BCA) and o-phthaldialdehyde (OPA) were used as coloring agents to specifically react with proteins and peptides. The results obtained with the BCA method and bovine serum albumin as standard did not correlate with the results obtained by the Kjeldahl method (r2 = 0.17). The OPA method showed better agreement with the Kjeldahl method (r2 = 0.69) and with the use of a whey protein hydrolysate as standard good estimates of the peptide contents in the hydrolysed milk protein samples was obtained compared to the results obtained with the Kjeldahl method.

U2 - 10.3923/ijds.2015.228.235

DO - 10.3923/ijds.2015.228.235

M3 - Journal article

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JO - International Journal of Dairy Science

JF - International Journal of Dairy Science

IS - 5

ER -

Comparative assessment of peptide concentration in milk protein hydrolysates and fractions

Abstract

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