Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2

Yajie Gao; Thomas C. de Bang; Jan K. Schjoerring

doi:10.1111/pbi.13046

Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO₂

Yajie Gao, Thomas C. de Bang, Jan K. Schjoerring^*

^*Corresponding author af dette arbejde

Plante- og Jordvidenskab

13 Citationer (Scopus)

26 Downloads (Pure)

Abstract

Cytosolic glutamine synthetase (GS1) plays a central role in nitrogen (N) metabolism. The importance of GS1 in N remobilization during reproductive growth has been reported in cereal species but attempts to improve N utilization efficiency (NUE) by overexpressing GS1 have yielded inconsistent results. Here, we demonstrate that transformation of barley (Hordeum vulgare L.) plants using a cisgenic strategy to express an extra copy of native HvGS1-1 lead to increased HvGS1.1 expression and GS1 enzyme activity. GS1 overexpressing lines exhibited higher grain yields and NUE than wild-type plants when grown under three different N supplies and two levels of atmospheric CO₂. In contrast with the wild-type, the grain protein concentration in the GS1 overexpressing lines did not decline when plants were exposed to elevated (800–900 μL/L) atmospheric CO₂. We conclude that an increase in GS1 activity obtained through cisgenic overexpression of HvGS1-1 can improve grain yield and NUE in barley. The extra capacity for N assimilation obtained by GS1 overexpression may also provide a means to prevent declining grain protein levels under elevated atmospheric CO₂.

Originalsprog	Engelsk
Tidsskrift	Plant Biotechnology Journal
Vol/bind	17
Udgave nummer	7
Sider (fra-til)	1209-1221
ISSN	1467-7644
DOI	https://doi.org/10.1111/pbi.13046
Status	Udgivet - jul. 2019

Adgang til dokumentet

10.1111/pbi.13046Licens: CC BY

Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2Forlagets udgivne version, 3,55 MBLicens: CC BY

Andre filer og links

Link to publication in Scopus

Citationsformater

Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO₂. / Gao, Yajie; de Bang, Thomas C.; Schjoerring, Jan K.
I: Plant Biotechnology Journal, Bind 17, Nr. 7, 07.2019, s. 1209-1221.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{27469976985f42c199ede3e6b00cc0ab,

title = "Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2",

abstract = "Cytosolic glutamine synthetase (GS1) plays a central role in nitrogen (N) metabolism. The importance of GS1 in N remobilization during reproductive growth has been reported in cereal species but attempts to improve N utilization efficiency (NUE) by overexpressing GS1 have yielded inconsistent results. Here, we demonstrate that transformation of barley (Hordeum vulgare L.) plants using a cisgenic strategy to express an extra copy of native HvGS1-1 lead to increased HvGS1.1 expression and GS1 enzyme activity. GS1 overexpressing lines exhibited higher grain yields and NUE than wild-type plants when grown under three different N supplies and two levels of atmospheric CO2. In contrast with the wild-type, the grain protein concentration in the GS1 overexpressing lines did not decline when plants were exposed to elevated (800–900 μL/L) atmospheric CO2. We conclude that an increase in GS1 activity obtained through cisgenic overexpression of HvGS1-1 can improve grain yield and NUE in barley. The extra capacity for N assimilation obtained by GS1 overexpression may also provide a means to prevent declining grain protein levels under elevated atmospheric CO2.",

keywords = "barley, carbon dioxide (CO), cisgenesis, glutamine synthetase (GS), grain protein, Nitrogen use efficiency (NUE)",

author = "Yajie Gao and {de Bang}, {Thomas C.} and Schjoerring, {Jan K.}",

year = "2019",

month = jul,

doi = "10.1111/pbi.13046",

language = "English",

volume = "17",

pages = "1209--1221",

journal = "Plant Biotechnology Journal",

issn = "1467-7644",

publisher = "Wiley-Blackwell",

number = "7",

}

TY - JOUR

T1 - Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2

AU - Gao, Yajie

AU - de Bang, Thomas C.

AU - Schjoerring, Jan K.

PY - 2019/7

Y1 - 2019/7

N2 - Cytosolic glutamine synthetase (GS1) plays a central role in nitrogen (N) metabolism. The importance of GS1 in N remobilization during reproductive growth has been reported in cereal species but attempts to improve N utilization efficiency (NUE) by overexpressing GS1 have yielded inconsistent results. Here, we demonstrate that transformation of barley (Hordeum vulgare L.) plants using a cisgenic strategy to express an extra copy of native HvGS1-1 lead to increased HvGS1.1 expression and GS1 enzyme activity. GS1 overexpressing lines exhibited higher grain yields and NUE than wild-type plants when grown under three different N supplies and two levels of atmospheric CO2. In contrast with the wild-type, the grain protein concentration in the GS1 overexpressing lines did not decline when plants were exposed to elevated (800–900 μL/L) atmospheric CO2. We conclude that an increase in GS1 activity obtained through cisgenic overexpression of HvGS1-1 can improve grain yield and NUE in barley. The extra capacity for N assimilation obtained by GS1 overexpression may also provide a means to prevent declining grain protein levels under elevated atmospheric CO2.

AB - Cytosolic glutamine synthetase (GS1) plays a central role in nitrogen (N) metabolism. The importance of GS1 in N remobilization during reproductive growth has been reported in cereal species but attempts to improve N utilization efficiency (NUE) by overexpressing GS1 have yielded inconsistent results. Here, we demonstrate that transformation of barley (Hordeum vulgare L.) plants using a cisgenic strategy to express an extra copy of native HvGS1-1 lead to increased HvGS1.1 expression and GS1 enzyme activity. GS1 overexpressing lines exhibited higher grain yields and NUE than wild-type plants when grown under three different N supplies and two levels of atmospheric CO2. In contrast with the wild-type, the grain protein concentration in the GS1 overexpressing lines did not decline when plants were exposed to elevated (800–900 μL/L) atmospheric CO2. We conclude that an increase in GS1 activity obtained through cisgenic overexpression of HvGS1-1 can improve grain yield and NUE in barley. The extra capacity for N assimilation obtained by GS1 overexpression may also provide a means to prevent declining grain protein levels under elevated atmospheric CO2.

KW - barley

KW - carbon dioxide (CO)

KW - cisgenesis

KW - glutamine synthetase (GS)

KW - grain protein

KW - Nitrogen use efficiency (NUE)

UR - http://www.scopus.com/inward/record.url?scp=85059140793&partnerID=8YFLogxK

U2 - 10.1111/pbi.13046

DO - 10.1111/pbi.13046

M3 - Journal article

C2 - 30525274

AN - SCOPUS:85059140793

SN - 1467-7644

VL - 17

SP - 1209

EP - 1221

JO - Plant Biotechnology Journal

JF - Plant Biotechnology Journal

IS - 7

ER -