Characterization of sequence variations in the D21S11 locus in Danes, Somalis and Greenlanders by second generation sequencing

TY - GEN

T1 - Characterization of sequence variations in the D21S11 locus in Danes, Somalis and Greenlanders by second generation sequencing

AU - Rockenbauer, Eszter

AU - Hansen, Stine

AU - Andersen, Malene Rask

AU - Heerup, Emilie

AU - Børsting, Claus

AU - Fordyce, Sarah Louise

AU - Morling, Niels

PY - 2013/10/31

Y1 - 2013/10/31

N2 - We used second generation sequencing to investigate the sequence variation and allele frequencies of D21S11 alleles in three geographically distant populations: Danes, Somalis and Greenlanders. Samples from 127 unrelated individuals (47 Danes, 57 Somalis and 23 Greenlanders) from Danish paternity and immigration cases were sequenced. In total, 36 allele variants with 14 different lengths were identified among the 256 alleles. The highest degree of variation was found in Somalis and the lowest one in Greenlanders. The number of repeats within the first and third variable repeat regions of the D21S11 locus had the highest variation between the samples investigated. This variation seemed to be population specific. The high sequence variation observed within the 127 subjects and within the three ethnic groups demonstrated the discriminatory power of the D21S11 STR system.

AB - We used second generation sequencing to investigate the sequence variation and allele frequencies of D21S11 alleles in three geographically distant populations: Danes, Somalis and Greenlanders. Samples from 127 unrelated individuals (47 Danes, 57 Somalis and 23 Greenlanders) from Danish paternity and immigration cases were sequenced. In total, 36 allele variants with 14 different lengths were identified among the 256 alleles. The highest degree of variation was found in Somalis and the lowest one in Greenlanders. The number of repeats within the first and third variable repeat regions of the D21S11 locus had the highest variation between the samples investigated. This variation seemed to be population specific. The high sequence variation observed within the 127 subjects and within the three ethnic groups demonstrated the discriminatory power of the D21S11 STR system.

M3 - Conference article

SN - 1875-1768

VL - 4

SP - e302-e303

JO - Forensic Science International: Genetics. Supplement Series

JF - Forensic Science International: Genetics. Supplement Series

IS - 1

T2 - 25’th congress of the International Society of Forensic Genetics

Y2 - 2 September 2013 through 7 September 2013

ER -