TY - JOUR
T1 - Characterization of bovine induced pluripotent stem cells by lentiviral transduction of reprogramming factor fusion proteins
AU - Cao, Hongguo
AU - Yang, Pan
AU - Pu, Yong
AU - Sun, Xueping
AU - Yin, Huiqun
AU - Zhang, Yu
AU - Zhang, Yunhai
AU - Li, Yunsheng
AU - Liu, Ya
AU - Fang, Fugui
AU - Zhang, Zijun
AU - Tao, Yong
AU - Zhang, Xiaorong
PY - 2012/3/21
Y1 - 2012/3/21
N2 - Pluripotent stem cells from domesticated animals have potential applications in transgenic breeding. Here, we describe induced pluripotent stem (iPS) cells derived from bovine fetal fibroblasts by lentiviral transduction of Oct4, Sox2, Klf4 and c-Myc defined-factor fusion proteins. Bovine iPS cells showed typical colony morphology, normal karyotypes, stained positively for alkaline phosphatase (AP) and expressed Oct4, Nanog and SSEA1. The CpG in the promoter regions of Oct4 and Nanog were highly unmethylated in bovine iPS cells compared to the fibroblasts. The cells were able to differentiate into cell types of all three germ layers in vitro and in vivo. In addition, these cells were induced into female germ cells under defined culture conditions and expressed early and late female germ cell-specific genes Vasa, Dazl, Gdf9, Nobox, Zp2, and Zp3. Our data suggest that bovine iPS cells were generated from bovine fetal fibroblasts with defined-factor fusion proteins mediated by lentivirus and have potential applications in bovine transgenic breeding and gene-modified animals.
AB - Pluripotent stem cells from domesticated animals have potential applications in transgenic breeding. Here, we describe induced pluripotent stem (iPS) cells derived from bovine fetal fibroblasts by lentiviral transduction of Oct4, Sox2, Klf4 and c-Myc defined-factor fusion proteins. Bovine iPS cells showed typical colony morphology, normal karyotypes, stained positively for alkaline phosphatase (AP) and expressed Oct4, Nanog and SSEA1. The CpG in the promoter regions of Oct4 and Nanog were highly unmethylated in bovine iPS cells compared to the fibroblasts. The cells were able to differentiate into cell types of all three germ layers in vitro and in vivo. In addition, these cells were induced into female germ cells under defined culture conditions and expressed early and late female germ cell-specific genes Vasa, Dazl, Gdf9, Nobox, Zp2, and Zp3. Our data suggest that bovine iPS cells were generated from bovine fetal fibroblasts with defined-factor fusion proteins mediated by lentivirus and have potential applications in bovine transgenic breeding and gene-modified animals.
KW - Alkaline Phosphatase
KW - Animals
KW - Cattle
KW - Cellular Reprogramming
KW - Fibroblasts
KW - Induced Pluripotent Stem Cells
KW - Kruppel-Like Transcription Factors
KW - Lentivirus
KW - Octamer Transcription Factor-3
KW - Proto-Oncogene Proteins c-myc
KW - SOXB1 Transcription Factors
KW - Transduction, Genetic
U2 - 10.7150/ijbs.3723
DO - 10.7150/ijbs.3723
M3 - Journal article
C2 - 22457605
SN - 1449-2288
VL - 8
SP - 498
EP - 511
JO - International Journal of Biological Sciences
JF - International Journal of Biological Sciences
IS - 4
ER -