TY - JOUR
T1 - Caleosins: Ca+2 binding proteins associated with oil-bodies
AU - Næsted, Henrik
AU - Frandsen, Gitte Inselmann
AU - Jauh, G.Y.
AU - Hernandez-Pinzon, I.
AU - Nielsen, Holger Frits Bech
AU - Murphy, D.J.
AU - Rogers, J.C.
AU - Mundy, John Williams
PY - 2000
Y1 - 2000
N2 - We have previously identified a rice gene encoding a 27 kDa protein with a single Ca2+-binding EF-hand and a putative membrane anchor. We report here similar genes termed caleosins, CLO, in other plants and fungi; they comprise a multigene family of at least five members in Arabidopsis (AtClo1-5). Northern hybridization demonstrated that AtClo2-4 mRNAs levels were low in various tissues, while AtClo1 mRNA levels were high in developing embryos and mature seeds. Analysis of transgenic Arabidopsis plants expressing the GUS reporter under control of the AtClo1 promoter showed strong levels of expression in developing embryos and also in root tip cells. Antibodies raised against AtCLO1 were used to detect caleosin in cellular fractions of Arabidopsis and rapeseed. This indicated that caleosins are a novel class of lipid body proteins, which may also be associated with an ER subdomain.
AB - We have previously identified a rice gene encoding a 27 kDa protein with a single Ca2+-binding EF-hand and a putative membrane anchor. We report here similar genes termed caleosins, CLO, in other plants and fungi; they comprise a multigene family of at least five members in Arabidopsis (AtClo1-5). Northern hybridization demonstrated that AtClo2-4 mRNAs levels were low in various tissues, while AtClo1 mRNA levels were high in developing embryos and mature seeds. Analysis of transgenic Arabidopsis plants expressing the GUS reporter under control of the AtClo1 promoter showed strong levels of expression in developing embryos and also in root tip cells. Antibodies raised against AtCLO1 were used to detect caleosin in cellular fractions of Arabidopsis and rapeseed. This indicated that caleosins are a novel class of lipid body proteins, which may also be associated with an ER subdomain.
U2 - 10.1023/A:1026564411918
DO - 10.1023/A:1026564411918
M3 - Journal article
SN - 0167-4412
VL - 44
SP - 463
EP - 476
JO - Plant Molecular Biology
JF - Plant Molecular Biology
IS - 4
ER -