Caleosins: Ca+2 binding proteins associated with oil-bodies

Henrik Næsted; Gitte Inselmann Frandsen; G.Y. Jauh; I. Hernandez-Pinzon; Holger Frits Bech Nielsen; D.J. Murphy; J.C. Rogers; John Williams Mundy

doi:10.1023/A:1026564411918

Caleosins: Ca⁺² binding proteins associated with oil-bodies

Henrik Næsted, Gitte Inselmann Frandsen, G.Y. Jauh, I. Hernandez-Pinzon, Holger Frits Bech Nielsen, D.J. Murphy, J.C. Rogers, John Williams Mundy

Centre m.v.

134 Citations (Scopus)

Abstract

We have previously identified a rice gene encoding a 27 kDa protein with a single Ca²⁺-binding EF-hand and a putative membrane anchor. We report here similar genes termed caleosins, CLO, in other plants and fungi; they comprise a multigene family of at least five members in Arabidopsis (AtClo1-5). Northern hybridization demonstrated that AtClo2-4 mRNAs levels were low in various tissues, while AtClo1 mRNA levels were high in developing embryos and mature seeds. Analysis of transgenic Arabidopsis plants expressing the GUS reporter under control of the AtClo1 promoter showed strong levels of expression in developing embryos and also in root tip cells. Antibodies raised against AtCLO1 were used to detect caleosin in cellular fractions of Arabidopsis and rapeseed. This indicated that caleosins are a novel class of lipid body proteins, which may also be associated with an ER subdomain.

Original language	English
Journal	Plant Molecular Biology
Volume	44
Issue number	4
Pages (from-to)	463-476
ISSN	0167-4412
DOIs	https://doi.org/10.1023/A:1026564411918
Publication status	Published - 2000

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title = "Caleosins: Ca+2 binding proteins associated with oil-bodies",

abstract = "We have previously identified a rice gene encoding a 27 kDa protein with a single Ca2+-binding EF-hand and a putative membrane anchor. We report here similar genes termed caleosins, CLO, in other plants and fungi; they comprise a multigene family of at least five members in Arabidopsis (AtClo1-5). Northern hybridization demonstrated that AtClo2-4 mRNAs levels were low in various tissues, while AtClo1 mRNA levels were high in developing embryos and mature seeds. Analysis of transgenic Arabidopsis plants expressing the GUS reporter under control of the AtClo1 promoter showed strong levels of expression in developing embryos and also in root tip cells. Antibodies raised against AtCLO1 were used to detect caleosin in cellular fractions of Arabidopsis and rapeseed. This indicated that caleosins are a novel class of lipid body proteins, which may also be associated with an ER subdomain.",

author = "Henrik N{\ae}sted and Frandsen, {Gitte Inselmann} and G.Y. Jauh and I. Hernandez-Pinzon and Nielsen, {Holger Frits Bech} and D.J. Murphy and J.C. Rogers and Mundy, {John Williams}",

year = "2000",

doi = "10.1023/A:1026564411918",

language = "English",

volume = "44",

pages = "463--476",

journal = "Plant Molecular Biology",

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T1 - Caleosins: Ca+2 binding proteins associated with oil-bodies

AU - Næsted, Henrik

AU - Frandsen, Gitte Inselmann

AU - Jauh, G.Y.

AU - Hernandez-Pinzon, I.

AU - Nielsen, Holger Frits Bech

AU - Murphy, D.J.

AU - Rogers, J.C.

AU - Mundy, John Williams

PY - 2000

Y1 - 2000

N2 - We have previously identified a rice gene encoding a 27 kDa protein with a single Ca2+-binding EF-hand and a putative membrane anchor. We report here similar genes termed caleosins, CLO, in other plants and fungi; they comprise a multigene family of at least five members in Arabidopsis (AtClo1-5). Northern hybridization demonstrated that AtClo2-4 mRNAs levels were low in various tissues, while AtClo1 mRNA levels were high in developing embryos and mature seeds. Analysis of transgenic Arabidopsis plants expressing the GUS reporter under control of the AtClo1 promoter showed strong levels of expression in developing embryos and also in root tip cells. Antibodies raised against AtCLO1 were used to detect caleosin in cellular fractions of Arabidopsis and rapeseed. This indicated that caleosins are a novel class of lipid body proteins, which may also be associated with an ER subdomain.

AB - We have previously identified a rice gene encoding a 27 kDa protein with a single Ca2+-binding EF-hand and a putative membrane anchor. We report here similar genes termed caleosins, CLO, in other plants and fungi; they comprise a multigene family of at least five members in Arabidopsis (AtClo1-5). Northern hybridization demonstrated that AtClo2-4 mRNAs levels were low in various tissues, while AtClo1 mRNA levels were high in developing embryos and mature seeds. Analysis of transgenic Arabidopsis plants expressing the GUS reporter under control of the AtClo1 promoter showed strong levels of expression in developing embryos and also in root tip cells. Antibodies raised against AtCLO1 were used to detect caleosin in cellular fractions of Arabidopsis and rapeseed. This indicated that caleosins are a novel class of lipid body proteins, which may also be associated with an ER subdomain.

U2 - 10.1023/A:1026564411918

DO - 10.1023/A:1026564411918

M3 - Journal article

SN - 0167-4412

VL - 44

SP - 463

EP - 476

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 4

ER -

Caleosins: Ca+2 binding proteins associated with oil-bodies

Abstract

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Caleosins: Ca⁺² binding proteins associated with oil-bodies