Building authentic midbrain dopaminergic neurons from stem CEllS - Lessons from development

TY - JOUR

T1 - Building authentic midbrain dopaminergic neurons from stem CEllS - Lessons from development

AU - Kirkeby, Agnete

AU - Parmar, Malin

PY - 2012/12/1

Y1 - 2012/12/1

N2 - The challenge with controlling the differentiation of human pluripotent cells to generate functional dopaminergic neurons for the treatment of Parkinson's disease has undergone significant progress in recent years. Here, we summarize the differences between newer and older protocols for generating midbrain dopaminergic neurons from human pluripotent stem cells, and we highlight the importance of following developmental pathways during differentiation. The field has now developed to a point where it is timely to take human pluripotent stem cells one step closer to clinical use, and cell criteria to be fulfilled for such developments are outlined in this review.

AB - The challenge with controlling the differentiation of human pluripotent cells to generate functional dopaminergic neurons for the treatment of Parkinson's disease has undergone significant progress in recent years. Here, we summarize the differences between newer and older protocols for generating midbrain dopaminergic neurons from human pluripotent stem cells, and we highlight the importance of following developmental pathways during differentiation. The field has now developed to a point where it is timely to take human pluripotent stem cells one step closer to clinical use, and cell criteria to be fulfilled for such developments are outlined in this review.

KW - Development

KW - Differentiation

KW - Dopaminergic neurons

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=84871421869&partnerID=8YFLogxK

U2 - 10.2478/s13380-012-0041-x

DO - 10.2478/s13380-012-0041-x

M3 - Review

AN - SCOPUS:84871421869

SN - 2081-3856

VL - 3

SP - 314

EP - 319

JO - Translational Neuroscience

JF - Translational Neuroscience

IS - 4

ER -