Antisense properties of peptide nucleic acid

H J Larsen; T Bentin; Peter E. Nielsen

Antisense properties of peptide nucleic acid

137 Citationer (Scopus)

Abstract

Peptide nucleic acid (PNA) is a nucleic acid mimic in which the deoxyribose phosphate backbone has been replaced by a pseudo-peptide polymer to which the nucleobases are linked. PNA-oligomers can be synthesized in relatively large amounts, are highly stable in biological environments, and bind complementary DNA and RNA targets with remarkably high affinity and specificity. Thus PNA possesses many of the properties desired for a good antisense agent. Until recently, limited uptake of PNA into cells has been the major obstacle for applying PNA as an antisense agent in cell cultures and in vivo. Here, the antisense properties of PNA in vitro and in vivo will be reviewed. In particular, we will focus on recent observations indicating that PNA equipped with or without various uptake moieties may function as an efficient and gene-specific inhibitor of translation in Escherichia coli and in certain mammalian cell types.

Originalsprog	Engelsk
Tidsskrift	B B A - Reviews on Cancer
Vol/bind	1489
Udgave nummer	1
Sider (fra-til)	159-66
Antal sider	8
ISSN	0006-3002
Status	Udgivet - 10 dec. 1999

Citationsformater

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title = "Antisense properties of peptide nucleic acid",

abstract = "Peptide nucleic acid (PNA) is a nucleic acid mimic in which the deoxyribose phosphate backbone has been replaced by a pseudo-peptide polymer to which the nucleobases are linked. PNA-oligomers can be synthesized in relatively large amounts, are highly stable in biological environments, and bind complementary DNA and RNA targets with remarkably high affinity and specificity. Thus PNA possesses many of the properties desired for a good antisense agent. Until recently, limited uptake of PNA into cells has been the major obstacle for applying PNA as an antisense agent in cell cultures and in vivo. Here, the antisense properties of PNA in vitro and in vivo will be reviewed. In particular, we will focus on recent observations indicating that PNA equipped with or without various uptake moieties may function as an efficient and gene-specific inhibitor of translation in Escherichia coli and in certain mammalian cell types.",

keywords = "Animals, Cell Membrane Permeability, Cells, Cultured, Drug Carriers/metabolism, Drug Stability, Escherichia coli/metabolism, Neurons/cytology, Nucleic Acid Hybridization/drug effects, Oligonucleotides, Antisense/chemistry, Oxytocin/antagonists & inhibitors, Peptide Nucleic Acids/chemistry, Protein Biosynthesis/drug effects, RNA, Messenger/antagonists & inhibitors, Rats, Receptors, Galanin, Receptors, Neuropeptide/antagonists & inhibitors, Receptors, Neurotensin/antagonists & inhibitors, Receptors, Opioid, mu/antagonists & inhibitors, Ribonuclease H/metabolism",

author = "Larsen, {H J} and T Bentin and Nielsen, {Peter E.}",

year = "1999",

month = dec,

day = "10",

language = "English",

volume = "1489",

pages = "159--66",

journal = "Biochimica et Biophysica Acta - Reviews on Cancer",

issn = "0304-419X",

publisher = "Elsevier",

number = "1",

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TY - JOUR

T1 - Antisense properties of peptide nucleic acid

AU - Larsen, H J

AU - Bentin, T

AU - Nielsen, Peter E.

PY - 1999/12/10

Y1 - 1999/12/10

N2 - Peptide nucleic acid (PNA) is a nucleic acid mimic in which the deoxyribose phosphate backbone has been replaced by a pseudo-peptide polymer to which the nucleobases are linked. PNA-oligomers can be synthesized in relatively large amounts, are highly stable in biological environments, and bind complementary DNA and RNA targets with remarkably high affinity and specificity. Thus PNA possesses many of the properties desired for a good antisense agent. Until recently, limited uptake of PNA into cells has been the major obstacle for applying PNA as an antisense agent in cell cultures and in vivo. Here, the antisense properties of PNA in vitro and in vivo will be reviewed. In particular, we will focus on recent observations indicating that PNA equipped with or without various uptake moieties may function as an efficient and gene-specific inhibitor of translation in Escherichia coli and in certain mammalian cell types.

AB - Peptide nucleic acid (PNA) is a nucleic acid mimic in which the deoxyribose phosphate backbone has been replaced by a pseudo-peptide polymer to which the nucleobases are linked. PNA-oligomers can be synthesized in relatively large amounts, are highly stable in biological environments, and bind complementary DNA and RNA targets with remarkably high affinity and specificity. Thus PNA possesses many of the properties desired for a good antisense agent. Until recently, limited uptake of PNA into cells has been the major obstacle for applying PNA as an antisense agent in cell cultures and in vivo. Here, the antisense properties of PNA in vitro and in vivo will be reviewed. In particular, we will focus on recent observations indicating that PNA equipped with or without various uptake moieties may function as an efficient and gene-specific inhibitor of translation in Escherichia coli and in certain mammalian cell types.

KW - Animals

KW - Cell Membrane Permeability

KW - Cells, Cultured

KW - Drug Carriers/metabolism

KW - Drug Stability

KW - Escherichia coli/metabolism

KW - Neurons/cytology

KW - Nucleic Acid Hybridization/drug effects

KW - Oligonucleotides, Antisense/chemistry

KW - Oxytocin/antagonists & inhibitors

KW - Peptide Nucleic Acids/chemistry

KW - Protein Biosynthesis/drug effects

KW - RNA, Messenger/antagonists & inhibitors

KW - Rats

KW - Receptors, Galanin

KW - Receptors, Neuropeptide/antagonists & inhibitors

KW - Receptors, Neurotensin/antagonists & inhibitors

KW - Receptors, Opioid, mu/antagonists & inhibitors

KW - Ribonuclease H/metabolism

M3 - Review

C2 - 10807005

SN - 0304-419X

VL - 1489

SP - 159

EP - 166

JO - Biochimica et Biophysica Acta - Reviews on Cancer

JF - Biochimica et Biophysica Acta - Reviews on Cancer

IS - 1

ER -

Antisense properties of peptide nucleic acid

Abstract

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