@article{797b27a05c1f11df928f000ea68e967b,
title = "ADD1/SREBP1c activates the PGC1-alpha promoter in brown adipocytes",
abstract = "Cold adaptation elicits a paradoxical simultaneous induction of fatty acid synthesis and β-oxidation in brown adipose tissue. We show here that cold exposure coordinately induced liver X receptor α (LXRα), adipocyte determination and differentiation-dependent factor 1 (ADD1)/sterol regulatory element-binding protein-1c (SREBP1c) and peroxisome proliferator-activated receptor γ coactivator-1α (PGC1α) in brown and inguinal white adipose tissues, but not in epididymal white adipose tissue. Using in vitro models of white and brown adipocytes we demonstrate that β-adrenergic stimulation induced expression of LXRα, ADD1/SREBP1c and PGC1α in cells with a brown-like adipose phenotype. We demonstrate that ADD1/SREBP1c is a powerful inducer of PGC1α expression via a conserved E box in the proximal promoter and that β-adrenergic stimulation led to recruitment of ADD1/SREBP1c to this E box. The ability of ADD1/SREBP1c to activate the PGC1α promoter exhibited a striking cell type dependency, suggesting that additional cell type-restricted factors contribute to ADD1/SREBP1c-mediated activation. In conclusion, our data demonstrate a novel role of ADD1/SREBP1c as a regulator of PGC1α expression in brown adipose tissue.",
author = "Qin Hao and Hansen, {Jacob B} and Petersen, {Rasmus K} and Philip Hallenborg and Claus J{\o}rgensen and Saverio Cinti and Larsen, {Philip J} and Steffensen, {Knut R} and Haibo Wang and Sheila Collins and Jun Wang and Jan-Ake Gustafsson and Lise Madsen and Karsten Kristiansen",
note = "Keywords: Adipocytes, Brown; Animals; Cell Differentiation; Cells, Cultured; Chromatin Immunoprecipitation; Electrophoretic Mobility Shift Assay; Electroporation; Male; Mice; Mice, Inbred C57BL; Orphan Nuclear Receptors; Promoter Regions, Genetic; RNA, Messenger; Regulatory Sequences, Nucleic Acid; Reverse Transcriptase Polymerase Chain Reaction; Sterol Regulatory Element Binding Protein 1; Trans-Activators",
year = "2010",
month = apr,
doi = "10.1016/j.bbalip.2009.11.008",
language = "English",
volume = "1801",
pages = "421--9",
journal = "BBA General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "4",
}