@article{af7985a0656611de8bc9000ea68e967b,
title = "A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis",
abstract = "A new method to test the sensitivity of tumour cells to chemotherapy is presented. Tumour cells were incubated in vitro on agar, and drug-induced cell cycle perturbation was monitored by flow cytometric DNA analysis. In the present study the method was applied to monitor the effect of adriamycin on an adriamycin-sensitive Ehrlich ascites tumour and two adriamycin-resistant tumours. Adriamycin caused a dose-related accumulation of tumour cells in the G2 + M phase in the sensitive tumour. Drug concentrations greater than or equal to 100-fold higher were required to induce similar changes in the resistant tumours. The dose level causing maximum accumulation in the G2 + M phase is suggested as a parameter for quantifying the sensitivity. The results indicate that the method can be extended to sensitivity testing of human tumours.",
author = "Engelholm, {S A} and M Spang-Thomsen and Vindel{\o}v, {L L}",
note = "Keywords: Animals; Carcinoma, Ehrlich Tumor; Cell Cycle; Cells, Cultured; DNA, Neoplasm; Dose-Response Relationship, Drug; Doxorubicin; Drug Resistance; Flow Cytometry; Mice",
year = "1983",
language = "English",
volume = "47",
pages = "497--502",
journal = "The British journal of cancer. Supplement",
issn = "0007-0920",
publisher = "nature publishing group",
number = "4",
}