A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis

S A Engelholm; M Spang-Thomsen; L L Vindeløv

A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis

S A Engelholm, M Spang-Thomsen, L L Vindeløv

16 Citations (Scopus)

Abstract

A new method to test the sensitivity of tumour cells to chemotherapy is presented. Tumour cells were incubated in vitro on agar, and drug-induced cell cycle perturbation was monitored by flow cytometric DNA analysis. In the present study the method was applied to monitor the effect of adriamycin on an adriamycin-sensitive Ehrlich ascites tumour and two adriamycin-resistant tumours. Adriamycin caused a dose-related accumulation of tumour cells in the G2 + M phase in the sensitive tumour. Drug concentrations greater than or equal to 100-fold higher were required to induce similar changes in the resistant tumours. The dose level causing maximum accumulation in the G2 + M phase is suggested as a parameter for quantifying the sensitivity. The results indicate that the method can be extended to sensitivity testing of human tumours.

Original language	English
Journal	British Journal of Cancer
Volume	47
Issue number	4
Pages (from-to)	497-502
Number of pages	5
ISSN	0007-0920
Publication status	Published - 1983

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@article{af7985a0656611de8bc9000ea68e967b,

title = "A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis",

abstract = "A new method to test the sensitivity of tumour cells to chemotherapy is presented. Tumour cells were incubated in vitro on agar, and drug-induced cell cycle perturbation was monitored by flow cytometric DNA analysis. In the present study the method was applied to monitor the effect of adriamycin on an adriamycin-sensitive Ehrlich ascites tumour and two adriamycin-resistant tumours. Adriamycin caused a dose-related accumulation of tumour cells in the G2 + M phase in the sensitive tumour. Drug concentrations greater than or equal to 100-fold higher were required to induce similar changes in the resistant tumours. The dose level causing maximum accumulation in the G2 + M phase is suggested as a parameter for quantifying the sensitivity. The results indicate that the method can be extended to sensitivity testing of human tumours.",

author = "Engelholm, {S A} and M Spang-Thomsen and Vindel{\o}v, {L L}",

note = "Keywords: Animals; Carcinoma, Ehrlich Tumor; Cell Cycle; Cells, Cultured; DNA, Neoplasm; Dose-Response Relationship, Drug; Doxorubicin; Drug Resistance; Flow Cytometry; Mice",

year = "1983",

language = "English",

volume = "47",

pages = "497--502",

journal = "The British journal of cancer. Supplement",

issn = "0007-0920",

publisher = "nature publishing group",

number = "4",

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TY - JOUR

T1 - A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis

AU - Engelholm, S A

AU - Spang-Thomsen, M

AU - Vindeløv, L L

N1 - Keywords: Animals; Carcinoma, Ehrlich Tumor; Cell Cycle; Cells, Cultured; DNA, Neoplasm; Dose-Response Relationship, Drug; Doxorubicin; Drug Resistance; Flow Cytometry; Mice

PY - 1983

Y1 - 1983

N2 - A new method to test the sensitivity of tumour cells to chemotherapy is presented. Tumour cells were incubated in vitro on agar, and drug-induced cell cycle perturbation was monitored by flow cytometric DNA analysis. In the present study the method was applied to monitor the effect of adriamycin on an adriamycin-sensitive Ehrlich ascites tumour and two adriamycin-resistant tumours. Adriamycin caused a dose-related accumulation of tumour cells in the G2 + M phase in the sensitive tumour. Drug concentrations greater than or equal to 100-fold higher were required to induce similar changes in the resistant tumours. The dose level causing maximum accumulation in the G2 + M phase is suggested as a parameter for quantifying the sensitivity. The results indicate that the method can be extended to sensitivity testing of human tumours.

AB - A new method to test the sensitivity of tumour cells to chemotherapy is presented. Tumour cells were incubated in vitro on agar, and drug-induced cell cycle perturbation was monitored by flow cytometric DNA analysis. In the present study the method was applied to monitor the effect of adriamycin on an adriamycin-sensitive Ehrlich ascites tumour and two adriamycin-resistant tumours. Adriamycin caused a dose-related accumulation of tumour cells in the G2 + M phase in the sensitive tumour. Drug concentrations greater than or equal to 100-fold higher were required to induce similar changes in the resistant tumours. The dose level causing maximum accumulation in the G2 + M phase is suggested as a parameter for quantifying the sensitivity. The results indicate that the method can be extended to sensitivity testing of human tumours.

M3 - Journal article

C2 - 6849794

SN - 0007-0920

VL - 47

SP - 497

EP - 502

JO - The British journal of cancer. Supplement

JF - The British journal of cancer. Supplement

IS - 4

ER -

A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis

Abstract

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