A proteomic characterization of factors enriched at nascent DNA molecules

TY - JOUR

T1 - A proteomic characterization of factors enriched at nascent DNA molecules

AU - Lopez-Contreras, Andres J

AU - Ruppen, Isabel

AU - Nieto-Soler, Maria

AU - Murga, Matilde

AU - Rodriguez-Acebes, Sara

AU - Remeseiro, Silvia

AU - Rodrigo-Perez, Sara

AU - Rojas, Ana M

AU - Mendez, Juan

AU - Muñoz, Javier

AU - Fernandez-Capetillo, Oscar

N1 - Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2013/4/25

Y1 - 2013/4/25

N2 - DNA replication is facilitated by multiple factors that concentrate in the vicinity of replication forks. Here, we developed an approach that combines the isolation of proteins on nascent DNA chains with mass spectrometry (iPOND-MS), allowing a comprehensive proteomic characterization of the human replisome and replisome-associated factors. In addition to known replisome components, we provide a broad list of proteins that reside in the vicinity of the replisome, some of which were not previously associated with replication. For instance, our data support a link between DNA replication and the Williams-Beuren syndrome and identify ZNF24 as a replication factor. In addition, we reveal that SUMOylation is widespread for factors that concentrate near replisomes, which contrasts with lower UQylation levels at these sites. This resource provides a panoramic view of the proteins that concentrate in the surroundings of the replisome, which should facilitate future investigations on DNA replication and genome maintenance.

AB - DNA replication is facilitated by multiple factors that concentrate in the vicinity of replication forks. Here, we developed an approach that combines the isolation of proteins on nascent DNA chains with mass spectrometry (iPOND-MS), allowing a comprehensive proteomic characterization of the human replisome and replisome-associated factors. In addition to known replisome components, we provide a broad list of proteins that reside in the vicinity of the replisome, some of which were not previously associated with replication. For instance, our data support a link between DNA replication and the Williams-Beuren syndrome and identify ZNF24 as a replication factor. In addition, we reveal that SUMOylation is widespread for factors that concentrate near replisomes, which contrasts with lower UQylation levels at these sites. This resource provides a panoramic view of the proteins that concentrate in the surroundings of the replisome, which should facilitate future investigations on DNA replication and genome maintenance.

KW - Cell Line

KW - DNA

KW - DNA Replication

KW - Gene Regulatory Networks

KW - HEK293 Cells

KW - Humans

KW - Kruppel-Like Transcription Factors

KW - Mass Spectrometry

KW - Proteomics

KW - Small Ubiquitin-Related Modifier Proteins

KW - Ubiquitination

KW - Williams Syndrome

U2 - 10.1016/j.celrep.2013.03.009

DO - 10.1016/j.celrep.2013.03.009

M3 - Journal article

C2 - 23545495

SN - 2211-1247

VL - 3

SP - 1105

EP - 1116

JO - Cell Reports

JF - Cell Reports

IS - 4

ER -