TY - JOUR
T1 - A porcine model for pathomorphological age assessment of surgically excised skin wounds
AU - Barington, Kristiane
AU - Dich-Jørgensen, Kristine
AU - Jensen, Henrik Elvang
PY - 2018
Y1 - 2018
N2 - A porcine model of second intention wound healing was established and gross and histological changes needed for accurate assessing the age of wounds were determined. Twenty-five pigs were anesthetized before incision of four wounds on each. The wounds were left to heal from 1 h to 35 days when the pigs were euthanized. In 14 pigs, biopsies were sampled from two wounds between days 2 and 18. By histological evaluation the following characteristics were found useful for determining the age of wounds: neutrophil:macrophage ratio, angiogenesis, hyperplasia of fibroblasts, presence of hemosiderophages and granulation tissue. The latter was present from day 4 (n = 8 wounds, 100%), but by gross evaluation it was not recognized until day 5 (n = 4 wounds, 100%). From day 4 to 10, the thickness of granulation tissue increased by 1.2 ± 2.4 mm/day. The thickness of collagen fibers within granulation tissue increased throughout the study period, and complete epithelization was reached from day 18.
AB - A porcine model of second intention wound healing was established and gross and histological changes needed for accurate assessing the age of wounds were determined. Twenty-five pigs were anesthetized before incision of four wounds on each. The wounds were left to heal from 1 h to 35 days when the pigs were euthanized. In 14 pigs, biopsies were sampled from two wounds between days 2 and 18. By histological evaluation the following characteristics were found useful for determining the age of wounds: neutrophil:macrophage ratio, angiogenesis, hyperplasia of fibroblasts, presence of hemosiderophages and granulation tissue. The latter was present from day 4 (n = 8 wounds, 100%), but by gross evaluation it was not recognized until day 5 (n = 4 wounds, 100%). From day 4 to 10, the thickness of granulation tissue increased by 1.2 ± 2.4 mm/day. The thickness of collagen fibers within granulation tissue increased throughout the study period, and complete epithelization was reached from day 18.
KW - Age of wounds
KW - Experimental animal model
KW - Pig
KW - Skin
KW - Ulceration
KW - Veterinary forensic pathology
U2 - 10.1186/s13028-018-0387-3
DO - 10.1186/s13028-018-0387-3
M3 - Journal article
C2 - 29848347
AN - SCOPUS:85047912525
SN - 0044-605X
VL - 60
JO - Acta Veterinaria Scandinavica
JF - Acta Veterinaria Scandinavica
IS - 1
M1 - 33
ER -