@article{eff6a9c002e111ddbee902004c4f4f50,
title = "A plant plasma membrane Ca2+ pump is required for normal pollen tube growth and fertilization.",
abstract = "Ca(2+) signals are thought to play important roles in plant growth and development, including key aspects of pollen tube growth and fertilization. The dynamics of a Ca(2+) signal are largely controlled by influx (through channels) and efflux (through pumps and antiporters). The Arabidopsis genome encodes 14 Ca(2+) pumps, 10 of which belong to a family of autoinhibited Ca(2+) ATPases (ACA) that are predicted to be activated by Ca(2+)/calmodulin. Here, we show that isoform ACA9 is expressed primarily in pollen and localized to the plasma membrane. Three independent T-DNA [portion of the Ti (tumor-inducing) plasmid that is transferred to plant cells] gene disruptions of ACA9 were found to result in partial male sterility. Complementation was observed by using a ACA9-yellow fluorescence protein (YFP) fusion that displayed plasma membrane localization. Mutant aca9 pollen displayed a reduced growth potential and a high frequency of aborted fertilization, resulting in a >80% reduction in seed set. These findings identify a plasma membrane Ca(2+) transporter as a key regulator of pollen development and fertilization in flowering plants.",
author = "Morten Schi{\o}tt and Romanowsky, {Shawn M} and Lone B{\ae}kgaard and Jakobsen, {Mia Kyed} and Michael Palmgren and Harper, {Jeffrey F}",
note = "Keywords: Arabidopsis; Calcium Signaling; Calcium-Transporting ATPases; Cell Membrane; Fertilization; Genome, Plant; Plasmids; Pollen",
year = "2004",
doi = "10.1073/pnas.0401542101",
language = "English",
volume = "101",
pages = "9502--7",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "25",
}