A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates

Eric Paul Bennett; Helle Hassan; Michael A. Hollingsworth; Henrik Clausen

doi:10.1016/S0014-5793(99)01268-5

A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates

Eric Paul Bennett, Helle Hassan, Michael A. Hollingsworth, Henrik Clausen^*

^*Corresponding author af dette arbejde

107 Citationer (Scopus)

Abstract

A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partially GalNAc-glycosylated acceptor substrates. GalNAc-T7 showed no activity with a large panel of non-glycosylated peptides, but was selectively activated by partial GalNAc glycosylation of peptide substrates derived from the tandem repeats of human MUC2 and rat submaxillary gland mucin. The function of GalNAc-T7 is suggested to be as a follow-up enzyme in the initiation step of O-glycosylation. Copyright (C) 1999 Federation of European Biochemical Societies.

Originalsprog	Engelsk
Tidsskrift	FEBS Letters
Vol/bind	460
Udgave nummer	2
Sider (fra-til)	226-230
Antal sider	5
ISSN	0014-5793
DOI	https://doi.org/10.1016/S0014-5793(99)01268-5
Status	Udgivet - 29 okt. 1999

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A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates. / Bennett, Eric Paul; Hassan, Helle; Hollingsworth, Michael A. et al.
I: FEBS Letters, Bind 460, Nr. 2, 29.10.1999, s. 226-230.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates",

abstract = "A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partially GalNAc-glycosylated acceptor substrates. GalNAc-T7 showed no activity with a large panel of non-glycosylated peptides, but was selectively activated by partial GalNAc glycosylation of peptide substrates derived from the tandem repeats of human MUC2 and rat submaxillary gland mucin. The function of GalNAc-T7 is suggested to be as a follow-up enzyme in the initiation step of O-glycosylation. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "GalNAc-transferase, Glycosyltransferase, Mucin, O-Glycosylation",

author = "Bennett, {Eric Paul} and Helle Hassan and Hollingsworth, {Michael A.} and Henrik Clausen",

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T1 - A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates

AU - Bennett, Eric Paul

AU - Hassan, Helle

AU - Hollingsworth, Michael A.

AU - Clausen, Henrik

PY - 1999/10/29

Y1 - 1999/10/29

N2 - A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partially GalNAc-glycosylated acceptor substrates. GalNAc-T7 showed no activity with a large panel of non-glycosylated peptides, but was selectively activated by partial GalNAc glycosylation of peptide substrates derived from the tandem repeats of human MUC2 and rat submaxillary gland mucin. The function of GalNAc-T7 is suggested to be as a follow-up enzyme in the initiation step of O-glycosylation. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partially GalNAc-glycosylated acceptor substrates. GalNAc-T7 showed no activity with a large panel of non-glycosylated peptides, but was selectively activated by partial GalNAc glycosylation of peptide substrates derived from the tandem repeats of human MUC2 and rat submaxillary gland mucin. The function of GalNAc-T7 is suggested to be as a follow-up enzyme in the initiation step of O-glycosylation. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - GalNAc-transferase

KW - Glycosyltransferase

KW - Mucin

KW - O-Glycosylation

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U2 - 10.1016/S0014-5793(99)01268-5

DO - 10.1016/S0014-5793(99)01268-5

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A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates

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