64Cu loaded liposomes as positron emission tomography imaging agents

Anncatrine Luisa Petersen; Tina Binderup; Palle Rasmussen; Jonas Rosager Henriksen; Dennis Ringkjøbing Elema; Andreas Kjær; Thomas Lars Andresen; Anncatrine Luisa Petersen; Tina Binderup; Palle Damkjær Rasmussen; Jonas Rosager Henriksen; Dennis Ringkjøbing Elema; Andreas Kjær; Thomas Lars Andresen

doi:10.1016/j.biomaterials.2010.11.059

64Cu loaded liposomes as positron emission tomography imaging agents

Anncatrine Luisa Petersen, Tina Binderup, Palle Rasmussen, Jonas Rosager Henriksen, Dennis Ringkjøbing Elema, Andreas Kjær, Thomas Lars Andresen, Anncatrine Luisa Petersen, Tina Binderup, Palle Damkjær Rasmussen, Jonas Rosager Henriksen, Dennis Ringkjøbing Elema, Andreas Kjær, Thomas Lars Andresen

Endocrinology and Metabolism

100 Citationer (Scopus)

Abstract

We have developed a highly efficient method for utilizing liposomes as imaging agents for positron emission tomography (PET) giving high resolution images and allowing direct quantification of tissue distribution and blood clearance. Our approach is based on remote loading of a copper-radionuclide ((64)Cu) using a new ionophore, 2-hydroxyquinoline, to carry (64)Cu(II) across the membrane of preformed liposomes and deliver it to an encapsulated copper-chelator. Using this ionophore we achieved very efficient loading (95.5 ± 1.6%) and retention stability (>99%), which makes the (64)Cu-liposomes highly applicable as PET imaging agents. We show the utility of the (64)Cu-liposomes for quantitative in vivo imaging of healthy and tumor-bearing mice using PET. This remote loading method is a powerful tool for characterizing the in vivo performance of liposome based nanomedicine, and has great potential in diagnostic and therapeutic applications.

Originalsprog	Engelsk
Tidsskrift	Biomaterials
Vol/bind	32
Udgave nummer	9
Sider (fra-til)	2334-41
Antal sider	8
ISSN	0142-9612
DOI	https://doi.org/10.1016/j.biomaterials.2010.11.059
Status	Udgivet - mar. 2011

Adgang til dokumentet

10.1016/j.biomaterials.2010.11.059

Citationsformater

Petersen, A. L., Binderup, T., Rasmussen, P., Henriksen, J. R., Elema, D. R., Kjær, A., Andresen, T. L., Petersen, A. L., Binderup, T., Rasmussen, P. D., Henriksen, J. R., Elema, D. R., Kjær, A., & Andresen, T. L. (2011). 64Cu loaded liposomes as positron emission tomography imaging agents. Biomaterials, 32(9), 2334-41. https://doi.org/10.1016/j.biomaterials.2010.11.059

@article{05b22a67bc114b25beaaf0a73fd84d9c,

title = "64Cu loaded liposomes as positron emission tomography imaging agents",

abstract = "We have developed a highly efficient method for utilizing liposomes as imaging agents for positron emission tomography (PET) giving high resolution images and allowing direct quantification of tissue distribution and blood clearance. Our approach is based on remote loading of a copper-radionuclide ((64)Cu) using a new ionophore, 2-hydroxyquinoline, to carry (64)Cu(II) across the membrane of preformed liposomes and deliver it to an encapsulated copper-chelator. Using this ionophore we achieved very efficient loading (95.5 ± 1.6%) and retention stability (>99%), which makes the (64)Cu-liposomes highly applicable as PET imaging agents. We show the utility of the (64)Cu-liposomes for quantitative in vivo imaging of healthy and tumor-bearing mice using PET. This remote loading method is a powerful tool for characterizing the in vivo performance of liposome based nanomedicine, and has great potential in diagnostic and therapeutic applications.",

keywords = "Animals, Calorimetry, Copper Radioisotopes, HT29 Cells, Humans, Ligands, Liposomes, Mice, Neoplasms, Particle Size, Positron-Emission Tomography, Static Electricity, Tissue Distribution",

author = "Petersen, {Anncatrine Luisa} and Tina Binderup and Palle Rasmussen and Henriksen, {Jonas Rosager} and Elema, {Dennis Ringkj{\o}bing} and Andreas Kj{\ae}r and Andresen, {Thomas Lars} and Petersen, {Anncatrine Luisa} and Tina Binderup and Rasmussen, {Palle Damkj{\ae}r} and Henriksen, {Jonas Rosager} and Elema, {Dennis Ringkj{\o}bing} and Andreas Kj{\ae}r and Andresen, {Thomas Lars}",

year = "2011",

month = mar,

doi = "10.1016/j.biomaterials.2010.11.059",

language = "English",

volume = "32",

pages = "2334--41",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier",

number = "9",

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TY - JOUR

T1 - 64Cu loaded liposomes as positron emission tomography imaging agents

AU - Petersen, Anncatrine Luisa

AU - Binderup, Tina

AU - Rasmussen, Palle

AU - Henriksen, Jonas Rosager

AU - Elema, Dennis Ringkjøbing

AU - Kjær, Andreas

AU - Andresen, Thomas Lars

AU - Petersen, Anncatrine Luisa

AU - Binderup, Tina

AU - Rasmussen, Palle Damkjær

AU - Henriksen, Jonas Rosager

AU - Elema, Dennis Ringkjøbing

AU - Kjær, Andreas

AU - Andresen, Thomas Lars

PY - 2011/3

Y1 - 2011/3

N2 - We have developed a highly efficient method for utilizing liposomes as imaging agents for positron emission tomography (PET) giving high resolution images and allowing direct quantification of tissue distribution and blood clearance. Our approach is based on remote loading of a copper-radionuclide ((64)Cu) using a new ionophore, 2-hydroxyquinoline, to carry (64)Cu(II) across the membrane of preformed liposomes and deliver it to an encapsulated copper-chelator. Using this ionophore we achieved very efficient loading (95.5 ± 1.6%) and retention stability (>99%), which makes the (64)Cu-liposomes highly applicable as PET imaging agents. We show the utility of the (64)Cu-liposomes for quantitative in vivo imaging of healthy and tumor-bearing mice using PET. This remote loading method is a powerful tool for characterizing the in vivo performance of liposome based nanomedicine, and has great potential in diagnostic and therapeutic applications.

AB - We have developed a highly efficient method for utilizing liposomes as imaging agents for positron emission tomography (PET) giving high resolution images and allowing direct quantification of tissue distribution and blood clearance. Our approach is based on remote loading of a copper-radionuclide ((64)Cu) using a new ionophore, 2-hydroxyquinoline, to carry (64)Cu(II) across the membrane of preformed liposomes and deliver it to an encapsulated copper-chelator. Using this ionophore we achieved very efficient loading (95.5 ± 1.6%) and retention stability (>99%), which makes the (64)Cu-liposomes highly applicable as PET imaging agents. We show the utility of the (64)Cu-liposomes for quantitative in vivo imaging of healthy and tumor-bearing mice using PET. This remote loading method is a powerful tool for characterizing the in vivo performance of liposome based nanomedicine, and has great potential in diagnostic and therapeutic applications.

KW - Animals

KW - Calorimetry

KW - Copper Radioisotopes

KW - HT29 Cells

KW - Humans

KW - Ligands

KW - Liposomes

KW - Mice

KW - Neoplasms

KW - Particle Size

KW - Positron-Emission Tomography

KW - Static Electricity

KW - Tissue Distribution

U2 - 10.1016/j.biomaterials.2010.11.059

DO - 10.1016/j.biomaterials.2010.11.059

M3 - Journal article

C2 - 21216003

SN - 0142-9612

VL - 32

SP - 2334

EP - 2341

JO - Biomaterials

JF - Biomaterials

IS - 9

ER -

64Cu loaded liposomes as positron emission tomography imaging agents

Abstract

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