Xenotransplantation of human neural progenitor cells to the subretinal space of nonimmunosuppressed pigs

Karin Warfvinge; Philip H Schwartz; Jens Folke Kiilgaard; Morten la Cour; Michael J Young; Erik Scherfig; Henry Klassen

doi:10.1155/2011/948740

Xenotransplantation of human neural progenitor cells to the subretinal space of nonimmunosuppressed pigs

Karin Warfvinge, Philip H Schwartz, Jens Folke Kiilgaard, Morten la Cour, Michael J Young, Erik Scherfig, Henry Klassen

Abstract

To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10-28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10-13 days and smaller numbers within the retina on days 12 and 13, with evidence suggesting a limited degree of morphological integration; however, no cells remained at 4 weeks. The strong mononuclear cell reaction and loss of donor cells indicate that modulation of host immunity is likely necessary for prolonged xenograft survival in this model.

Original language	English
Journal	Journal of Transplantation
Volume	2011
Pages (from-to)	948740
ISSN	2090-0007
DOIs	https://doi.org/10.1155/2011/948740
Publication status	Published - 2011

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title = "Xenotransplantation of human neural progenitor cells to the subretinal space of nonimmunosuppressed pigs",

abstract = "To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10-28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10-13 days and smaller numbers within the retina on days 12 and 13, with evidence suggesting a limited degree of morphological integration; however, no cells remained at 4 weeks. The strong mononuclear cell reaction and loss of donor cells indicate that modulation of host immunity is likely necessary for prolonged xenograft survival in this model.",

author = "Karin Warfvinge and Schwartz, {Philip H} and Kiilgaard, {Jens Folke} and {la Cour}, Morten and Young, {Michael J} and Erik Scherfig and Henry Klassen",

year = "2011",

doi = "10.1155/2011/948740",

language = "English",

volume = "2011",

pages = "948740",

journal = "Journal of Transplantation",

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TY - JOUR

T1 - Xenotransplantation of human neural progenitor cells to the subretinal space of nonimmunosuppressed pigs

AU - Warfvinge, Karin

AU - Schwartz, Philip H

AU - Kiilgaard, Jens Folke

AU - la Cour, Morten

AU - Young, Michael J

AU - Scherfig, Erik

AU - Klassen, Henry

PY - 2011

Y1 - 2011

N2 - To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10-28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10-13 days and smaller numbers within the retina on days 12 and 13, with evidence suggesting a limited degree of morphological integration; however, no cells remained at 4 weeks. The strong mononuclear cell reaction and loss of donor cells indicate that modulation of host immunity is likely necessary for prolonged xenograft survival in this model.

AB - To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10-28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10-13 days and smaller numbers within the retina on days 12 and 13, with evidence suggesting a limited degree of morphological integration; however, no cells remained at 4 weeks. The strong mononuclear cell reaction and loss of donor cells indicate that modulation of host immunity is likely necessary for prolonged xenograft survival in this model.

U2 - 10.1155/2011/948740

DO - 10.1155/2011/948740

M3 - Journal article

SN - 2090-0007

VL - 2011

SP - 948740

JO - Journal of Transplantation

JF - Journal of Transplantation

ER -

Xenotransplantation of human neural progenitor cells to the subretinal space of nonimmunosuppressed pigs

Abstract

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